用光声显微镜对急性热烧伤进行成像。

Imaging acute thermal burns by photoacoustic microscopy.

作者信息

Zhang Hao F, Maslov Konstantin, Stoica George, Wang Lihong V

机构信息

Department of Biomedical Engineering, Optical Imaging Laboratory, Texas A&M University, 3120 TAMU, College Station, Texas 77843-3120, USA.

出版信息

J Biomed Opt. 2006 Sep-Oct;11(5):054033. doi: 10.1117/1.2355667.

DOI:10.1117/1.2355667

PMID:17092182

Abstract

The clinical significance of a burn depends on the percentage of total body involved and the depth of the burn. Hence a noninvasive method that is able to evaluate burn depth would be of great help in clinical evaluation. To this end, photoacoustic microscopy is used to determine the depth of acute thermal burns by imaging the total hemoglobin concentration in the blood that accumulates along the boundaries of injuries as a result of thermal damage to the vasculature. We induce acute thermal burns in vivo on pig skin with cautery. Photoacoustic images of the burns are acquired after skin excision. In a burn treated at 175 degrees C for 20 s, the maximum imaged burn depth is 1.73+/-0.07 mm. In burns treated at 150 degrees C for 5, 10, 20, and 30 s, respectively, the trend of increasing maximum burn depth with longer thermal exposure is demonstrated.

摘要

烧伤的临床意义取决于全身受累的百分比和烧伤深度。因此，一种能够评估烧伤深度的非侵入性方法对临床评估将有很大帮助。为此，光声显微镜通过对因血管热损伤而在损伤边界处积聚的血液中的总血红蛋白浓度进行成像，来确定急性热烧伤的深度。我们用烧灼法在猪皮肤上进行体内急性热烧伤。皮肤切除后获取烧伤的光声图像。在175摄氏度下处理20秒的烧伤中，成像的最大烧伤深度为1.73±0.07毫米。在分别于150摄氏度下处理5、10、20和30秒的烧伤中，显示出随着热暴露时间延长最大烧伤深度增加的趋势。