Paul-Prasanth Bindhu, Matsuda Masaru, Lau En-Lieng, Suzuki Aya, Sakai Fumie, Kobayashi Tohru, Nagahama Yoshitaka
Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan.
Biochem Biophys Res Commun. 2006 Dec 29;351(4):815-9. doi: 10.1016/j.bbrc.2006.10.095. Epub 2006 Oct 30.
DMY is the second vertebrate sex-determining gene identified from the fish, Oryzias latipes. In this study, we used two different ways of sex reversal, DMY knock-down and estradiol-17beta (E2) treatment, to determine the possible function of DMY during early gonadal sex differentiation in XY medaka. Our findings revealed that the mitotic and meiotic activities of the germ cells in the 0 day after hatching (dah) DMY knock-down XY larvae were identical to those of the normal XX larvae, suggesting the microenvironment of these XY gonads to be similar to that of the normal XX gonad, where DMY is naturally absent. Conversely, E2 treatment failed to initiate mitosis in the XY gonad, possibly due to an active DMY, even though it could initiate meiosis. Present study is the first to prove that the germ cells in the XY gonad can resume the mitotic activity, if DMY was knocked down.
DMY是从青鳉鱼（Oryzias latipes）中鉴定出的第二个脊椎动物性别决定基因。在本研究中，我们采用了两种不同的性逆转方法，即敲低DMY和用17β-雌二醇（E2）处理，以确定DMY在XY青鳉早期性腺性别分化过程中的可能功能。我们的研究结果表明，孵化后0天（dah）敲低DMY的XY幼虫生殖细胞的有丝分裂和减数分裂活动与正常XX幼虫相同，这表明这些XY性腺的微环境与正常XX性腺相似，而正常XX性腺中天然不存在DMY。相反，E2处理未能在XY性腺中启动有丝分裂，这可能是由于DMY的活性，尽管它可以启动减数分裂。本研究首次证明，如果敲低DMY，XY性腺中的生殖细胞可以恢复有丝分裂活性。
