Ferlazzo G, Scisca C, Iemmo R, Quartarone G, Cicciarello R, Gagliardi M E, Mesiti M
Sezione Decentrata di Messina and Istituto di Oncologia, Universita'di Messina, Italy.
J Urol. 1997 Jan;157(1):366-70.
Previous studies have reported that high energy shock waves (HESW), generated by an electrohydraulic lithotriptor, may have some utility as a cancer treatment modality. Furthermore, it has been described that shock waves propagating in a fluid, show demolitive effects at the level of the interface of a solid fragment immersed in the fluid. In this study, we demonstrate that it is possible to enhance the antineoplastic effects of HESW if treated cells or tissues are linked to monoclonal antibodies (MoAbs) conjugated with metallic beads (MB) (about 1 mu of diameter) and specific for a cancer cell surface determinant. A leukemic cell line was used to study the effects of HESW on cells linked to MB. A fresh human breast cancer specimen was used to perform the assay on tumor tissue. MB linked treated cell viability, growth curve, cloning efficiency and Bromodeoxyuridine incorporation were reduced in comparison to cells treated with HESW alone. Our data suggest that the presence of solid fragments vehicled by MoAbs on a cancer cell surface is able to synergize with the limited antineoplastic effects of HESW.
先前的研究报道，由液电碎石机产生的高能冲击波（HESW）可能作为一种癌症治疗方式具有一定作用。此外，据描述，在流体中传播的冲击波在浸没于流体中的固体碎片界面处显示出破坏作用。在本研究中，我们证明，如果将处理过的细胞或组织与结合有金属珠（MB，直径约1微米）且对癌细胞表面决定簇具有特异性的单克隆抗体（MoAb）相连接，就有可能增强HESW的抗肿瘤作用。使用一种白血病细胞系来研究HESW对与MB相连的细胞的作用。用一份新鲜的人乳腺癌标本对肿瘤组织进行检测。与仅用HESW处理的细胞相比，与MB相连处理后的细胞活力、生长曲线、克隆效率和溴脱氧尿苷掺入均降低。我们的数据表明，由MoAb携带的固体碎片存在于癌细胞表面能够与HESW有限的抗肿瘤作用协同发挥作用。
