Zhang Xiao Dong, Deslandes Edwige, Villedieu Marie, Poulain Laurent, Duval Marilyne, Gauduchon Pascal, Schwartz Laurent, Icard Philippe
Groupe Régional d'Etudes sur le Cancer (Grecan), Unité "Biologie et Thérapies Innovantes des Cancers Localement Agressifs", CLCC François Baclesse, Caen, France.
Anticancer Res. 2006 Sep-Oct;26(5A):3561-6.
2-Deoxy-D-glucose (2-DG) is an analog of glucose that is preferentially captured by tumors and is accumulated in transformed cells, because the phosphorylated molecule (2-DG-6P) cannot be metabolized or diffused outside the cells. Targeted with a fluorine atom, 18F-DG is currently used to visualize malignant tumors (PET scan). Although cancer cells have been reported to be strongly dependent on glycolysis (Warburg effect), very few reports have studied the inhibitory effects of 2-DG on cancer.
Our objective was to study, in a large panel of human malignant cells of various origins (ovarian, squamous, cerebral, hepatic, colonic and mesothelial), if the inhibitory activity of 2DG against tumor growth could be considered a general phenomenon and to determine its effect on the cell cycle.
Four types of response in the different cell lines were observed when cells were cultured in the presence of 2-DG (5 mM) continuous exposure: proliferation slow down; proliferation arrest without signs of apoptosis; strong cell cycle arrest accompanied by moderate apoptosis induction; massive apoptosis.
2-DG appears as an interesting new therapeutic agent against cancer in vitro, and should be tested in in vivo studies.
2-脱氧-D-葡萄糖（2-DG）是葡萄糖的类似物，肿瘤会优先摄取它并在转化细胞中蓄积，因为磷酸化分子（2-DG-6P）无法在细胞外代谢或扩散。18F-DG通过氟原子标记，目前用于可视化恶性肿瘤（PET扫描）。尽管据报道癌细胞强烈依赖糖酵解（瓦伯格效应），但很少有报告研究2-DG对癌症的抑制作用。
我们的目的是在大量源自不同组织（卵巢、鳞状、脑、肝、结肠和间皮）的人类恶性细胞中研究，2-DG对肿瘤生长的抑制活性是否可被视为一种普遍现象，并确定其对细胞周期的影响。
当细胞在2-DG（5 mM）持续暴露下培养时，在不同细胞系中观察到四种类型的反应：增殖减缓；增殖停滞且无凋亡迹象；强烈的细胞周期停滞并伴有中度凋亡诱导；大量凋亡。
2-DG在体外似乎是一种有前景的新型抗癌治疗药物，应在体内研究中进行测试。
