Yasuda Manabu, Mizukami Makiko, Hanagiri Takeshi, Shigematsu Yoshiki, Fukuyama Takashi, Nagata Yoshika, So Tetsuya, Ichiki Yoshinobu, Sugaya Masakazu, Takenoyama Mitsuhiro, Sugio Kenji, Yasumoto Kosei
Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Anticancer Res. 2006 Sep-Oct;26(5A):3607-11.
Lung cancer tissues are often infiltrated by B lymphocytes, but it is not clear whether these infiltrations represent tumor-specific immune response or a nonspecific reaction.
The serological analysis of recombinant cDNA expression libraries (SEREX) were previously modified using a severe combined immunodeficient (SCID) mice model engrafted with fresh human lung cancer. Here, a panel of antigens recognized by tumor-infiltrating B lymphocytes (TIB) in human lung cancer were characterized.
The modified SEREX analysis identified 22 distinct antigens in a large cell carcinoma of the lung. Sequence analysis and real time-PCR analysis showed that 55% of isolated antigens were overexpressed in tumor tissues and 9% had mutation.
The results of this study indicate that the humoral immune response of TIB in lung cancer patients can be detected in the xenotransplanted SCID mouse model and our modification shows high sensitivity and specificity for identification of tumor antigens.
肺癌组织常被B淋巴细胞浸润，但尚不清楚这些浸润是代表肿瘤特异性免疫反应还是非特异性反应。
先前利用植入新鲜人肺癌的严重联合免疫缺陷（SCID）小鼠模型对重组cDNA表达文库的血清学分析（SEREX）进行了改良。在此，对人肺癌中肿瘤浸润性B淋巴细胞（TIB）识别的一组抗原进行了表征。
改良的SEREX分析在肺大细胞癌中鉴定出22种不同抗原。序列分析和实时PCR分析表明，55%的分离抗原在肿瘤组织中过表达，9%发生突变。
本研究结果表明，在异种移植的SCID小鼠模型中可检测到肺癌患者TIB的体液免疫反应，且我们的改良方法在鉴定肿瘤抗原方面具有高灵敏度和特异性。
