在锤头状核酶切割机制研究中构型明确的含硫代磷酸酯寡核糖核苷酸
Configurationally defined phosphorothioate-containing oligoribonucleotides in the study of the mechanism of cleavage of hammerhead ribozymes.
作者信息
Slim G, Gait M J
机构信息
MRC Laboratory of Molecular Biology, Cambridge, UK.
出版信息
Nucleic Acids Res. 1991 Mar 25;19(6):1183-8. doi: 10.1093/nar/19.6.1183.
Abstract
The chemical synthesis is described of oligoribonucleotides containing a single phosphorothioate linkage of defined Rp and Sp configuration. The oligoribonucleotides were used as substrates in the study of the mechanism of cleavage of an RNA hammerhead domain having the phosphorothioate group at the cleavage site. Whereas the Rp isomer was cleaved only very slowly in the presence of magnesium ion, the rate of cleavage of the Sp isomer was only slightly reduced from that of the unmodified phosphodiester. This finding gives further evidence for the hypothesis that the magnesium ion is bound to the pro-R oxygen in the transition state of the hammerhead cleavage reaction. Also, inversion of configuration at phosphorus is confirmed for a two-stranded hammerhead.
摘要
本文描述了含有特定Rp和Sp构型的单个硫代磷酸酯键的寡核糖核苷酸的化学合成。这些寡核糖核苷酸被用作底物，用于研究在切割位点具有硫代磷酸酯基团的RNA锤头结构域的切割机制。在镁离子存在下，Rp异构体的切割非常缓慢，而Sp异构体的切割速率与未修饰的磷酸二酯相比仅略有降低。这一发现进一步证明了以下假设：在锤头切割反应的过渡态中，镁离子与前-R氧结合。此外，对于双链锤头，磷原子的构型翻转也得到了证实。
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