Jeffries A C, Symons R H
Department of Biochemistry, University of Adelaide, Australia.
Nucleic Acids Res. 1989 Feb 25;17(4):1371-7. doi: 10.1093/nar/17.4.1371.
A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+. The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-menten kinetic parameters for the reaction were; Km 1.3 microM, Vmax 0.012 microM min-1, kcat 0.5 min-1. The 13-mer RNA is the smallest ribozyme so far reported. A DNA analogue of the 13-mer can not substitute for the RNA in the reaction.
一个13聚体的寡核糖核苷酸在Mg2+存在的情况下，可以作为一种核酶，对一个41聚体的寡核糖核苷酸底物进行特异性的自我切割。所涉及的两个序列对应于苜蓿暂时条纹病毒类病毒的自我切割锤头结构。该反应的米氏动力学参数为：Km 1.3微摩尔，Vmax 0.012微摩尔/分钟，kcat 0.5分钟-1。这个13聚体RNA是迄今为止报道的最小的核酶。13聚体的DNA类似物在该反应中不能替代RNA。
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