Kitson J D, Burke K L, Pullen L A, Belsham G J, Almond J W
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, United Kingdom.
J Virol. 1991 Jun;65(6):3068-75. doi: 10.1128/JVI.65.6.3068-3075.1991.
Five poliovirus recombinants containing sequences corresponding to foot-and-mouth disease virus (FMDV) antigenic sites were constructed. Viable virus was recovered from four of these plasmids, in which the VP1 beta B-beta C loop (antigenic site 1) of poliovirus type 1 Sabin had been replaced with sequences derived from the VP1 beta G-beta H loop (antigenic site 1) of FMDV O1 Kaufbeuren (O1K), chimera O1.1 (residues 141 to 154), chimera O1.2 (residues 147 to 156), and chimera O1.3 (residues 140 to 160) or from the beta B-beta C loop of VP1 (antigenic site 3) in chimera O3.1 (residues 40 to 49). One chimera (O1.3) was neutralized by FMDV-specific polyclonal serum and monoclonal antibodies directed against antigenic site 1 of FMDV. Chimeras O1.3 and O3.1 induced site-specific FMDV-neutralizing antibodies in guinea pigs. Chimera O1.3 was capable of inducing a protective response against FMDV challenge in some guinea pigs.
构建了5种含有与口蹄疫病毒（FMDV）抗原位点相对应序列的脊髓灰质炎病毒重组体。从其中4种质粒中回收了活病毒，其中1型脊髓灰质炎病毒Sabin株的VP1 βB-βC环（抗原位点1）已被源自FMDV O1 Kaufbeuren（O1K）、嵌合体O1.1（第141至154位氨基酸）、嵌合体O1.2（第147至156位氨基酸）和嵌合体O1.3（第140至160位氨基酸）的VP1 βG-βH环（抗原位点1）的序列所取代，或者被嵌合体O3.1（第40至49位氨基酸）中VP1的βB-βC环（抗原位点3）所取代。一种嵌合体（O1.3）被FMDV特异性多克隆血清和针对FMDV抗原位点1的单克隆抗体所中和。嵌合体O1.3和O3.1在豚鼠中诱导了位点特异性的FMDV中和抗体。嵌合体O1.3能够在一些豚鼠中诱导针对FMDV攻击的保护性反应。
