Honda Masahiko, Kurisaki Akira, Ohnuma Kiyoshi, Okochi Hitoshi, Hamazaki Tatsuo S, Asashima Makoto
Department of Biological Sciences, Graduate School of Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Biochem Biophys Res Commun. 2006 Dec 29;351(4):877-82. doi: 10.1016/j.bbrc.2006.10.126. Epub 2006 Nov 2.
Cardiomyocytes are known to differentiate spontaneously from embryonic stem (ES) cells when they formed aggregates, so called "embryoid bodies", in the presence of serum. In this study, we explored the induction of cardiomyocytes from mouse ES cells in chemically defined serum-free medium by using a mesoderm-inducing factor, BMP4. Comparing the different inductive methods, we found a candidate cell surface marker, N-cadherin, for cardiomyocyte progenitors from ES cells. N-cadherin-positive cells highly expressed cardiogenic markers, Nkx2.5, Tbx5, and Isl1, and showed a high differentiation rate into cardiomyocyte lineage. These results indicate that N-cadherin can be a useful cell surface marker for the progenitors of cardiomyocyte differentiated from ES cells in the serum-free culture.
已知当胚胎干细胞（ES细胞）在血清存在的情况下形成聚集体，即所谓的“胚状体”时，它们会自发分化为心肌细胞。在本研究中，我们通过使用中胚层诱导因子BMP4，探索了在化学成分明确的无血清培养基中从小鼠ES细胞诱导生成心肌细胞的方法。通过比较不同的诱导方法，我们发现了一种候选细胞表面标志物N-钙黏蛋白，用于鉴定ES细胞来源的心肌细胞祖细胞。N-钙黏蛋白阳性细胞高表达心脏发生标志物Nkx2.5、Tbx5和Isl1，并显示出向心肌细胞谱系的高分化率。这些结果表明，在无血清培养中，N-钙黏蛋白可以作为ES细胞分化而来的心肌细胞祖细胞的一种有用的细胞表面标志物。
