Marchand C, Druilhe P
Parasitologie Biomédicale, Institut Pasteur, Paris, France.
Bull World Health Organ. 1990;68 Suppl(Suppl):158-64.
The restricted access to Plasmodium falciparum liver stages has greatly limited the analysis of the antigenic content of that stage. Due to the lack of material to perform immunochemical studies, of access to mRNA, and of monoclonal probes, we decided to screen a genomic library with stage-restricted human antibodies. This strategy led to the identification of a large number of DNA fragments encoding both sporozoite specific as well as liver-stage specific epitopes. Following the initial characterization of one liver-stage antigen, further screening was performed by using additional selective human antibodies. These were defined as having a high degree of reactivity with native antigens on either of the two stages while being negative with the already known molecules of the two stages. From this second screening and the study of cross-reactions, several subsets of DNA clones expressing antigens present on the surface of sporozoites, or in liver stages, or in both, could be identified. In exposed individuals a high prevalence of antibodies to several of these antigens was found.
对恶性疟原虫肝期的研究受限，这极大地限制了对该阶段抗原成分的分析。由于缺乏用于进行免疫化学研究的材料、获取mRNA的途径以及单克隆探针，我们决定用阶段特异性人抗体筛选基因组文库。这一策略导致鉴定出大量编码子孢子特异性和肝期特异性表位的DNA片段。在初步鉴定一种肝期抗原之后，使用额外的选择性人抗体进行了进一步筛选。这些抗体被定义为与两个阶段的天然抗原具有高度反应性，而与两个阶段已知分子呈阴性反应。通过第二次筛选和交叉反应研究，可以鉴定出几个表达存在于子孢子表面、肝期或两者中的抗原的DNA克隆亚群。在暴露个体中，发现针对其中几种抗原的抗体具有高流行率。
