Galey B, Druilhe P, Ploton I, Desgranges C, Asavanich A, Harinasuta T, Marchand C, Brahimi K, Charoenvit Y, Paul C
Laboratoire de Parasitologie Bio-Medicale, Institut Pasteur, Paris, France.
Infect Immun. 1990 Sep;58(9):2995-3001. doi: 10.1128/iai.58.9.2995-3001.1990.
We have compared the reactivities of antibodies developed by individuals frequently exposed to Plasmodium falciparum infections with the epitopes contained within the repeats of the circumsporozoite (CS) protein and their reactivities with the epitopes of a native molecule(s) accessible on the sporozoite surface. Results of direct-binding assays and competition assays between artificial and native molecules or between human antibodies and anti-CS monoclonal antibodies suggest that humans respond preferentially to epitopes not contained within the repeats of the CS protein and probably not contained in the whole CS protein. Human monoclonal antibodies reactive with P. falciparum sporozoite surface antigens were produced by Epstein-Barr virus transformation of human lymphocytes. Their pattern of reactivity with sporozoites from a number of different isolates indicates the existence of several distinct epitopes on the parasite surface. Differences between isolates and between sporozoites within a given sample were observed. No single human monoclonal antibody capable of detecting an epitope expressed in all the parasites studied was found.
我们比较了经常暴露于恶性疟原虫感染的个体所产生的抗体与环子孢子（CS）蛋白重复序列中所含表位的反应性，以及它们与子孢子表面可及的天然分子表位的反应性。人工分子与天然分子之间或人抗体与抗CS单克隆抗体之间的直接结合试验和竞争试验结果表明，人类优先对CS蛋白重复序列中不包含且可能整个CS蛋白中也不包含的表位作出反应。通过爱泼斯坦 - 巴尔病毒转化人淋巴细胞产生了与恶性疟原虫子孢子表面抗原反应的人单克隆抗体。它们与来自多个不同分离株的子孢子的反应模式表明寄生虫表面存在几种不同的表位。观察到不同分离株之间以及给定样本中子孢子之间的差异。未发现能够检测在所研究的所有寄生虫中表达的表位的单一人类单克隆抗体。
