Tobias Dennis J, Manoharan Muthusamy, Pritsch Clara, Dahleen Lynn S
Department of Plant Sciences, North Dakota State University, Fargo, ND 58105, USA.
Plant Cell Rep. 2007 May;26(5):631-9. doi: 10.1007/s00299-006-0263-x. Epub 2006 Nov 11.
Pathogenesis-related (PR) proteins associated with degradation of structural components of pathogenic filamentous fungi were overexpressed in the two-rowed malting barley (Hordeum vulgare L.) cultivar Conlon. Transgenes were introduced by co-bombardment with two plasmids, one carrying a rice (Oryza sativa L.) chitinase gene (chi11) and another carrying a rice thaumatin-like protein gene (tlp). Each gene was under the control of the maize ubiquitin (Ubi1) promoter. Fifty-eight primary transformants from three independent transformation events were regenerated. T(1) plants with high rice chi11 and tlp protein expression levels were advanced to identify T(2) homozygotes by herbicide spray and subjected to further molecular analyses. T(3) progeny from one event (E2) had stable integration and expression of the rice chi11 and tlp while those from the other events (E1 and E3) showed stable integration only of tlp. The successful production of these lines overexpressing the antifungal chi and tlp proteins provides materials to test the effects of these genes on a variety of fungal diseases that attack barley and to serve as potential additional sources of disease resistance.
与致病丝状真菌结构成分降解相关的病程相关（PR）蛋白在二棱制麦大麦（Hordeum vulgare L.）品种Conlon中过表达。通过与两个质粒共轰击导入转基因，一个携带水稻（Oryza sativa L.）几丁质酶基因（chi11），另一个携带水稻类甜蛋白基因（tlp）。每个基因都受玉米泛素（Ubi1）启动子的控制。从三个独立转化事件中再生出58个初级转化体。通过除草剂喷雾筛选出水稻chi11和tlp蛋白表达水平高的T(1)植株，以鉴定T(2)纯合子，并进行进一步的分子分析。来自一个事件（E2）的T(3)后代具有水稻chi11和tlp的稳定整合和表达，而来自其他事件（E1和E3）的后代仅显示tlp的稳定整合。这些过表达抗真菌chi和tlp蛋白的品系的成功产生，为测试这些基因对攻击大麦的多种真菌病害的影响提供了材料，并作为潜在的额外抗病来源。
