Gkizi Danai, Poulaki Eirini G, Tjamos Sotirios E
Laboratory of Plant Pathology, Agricultural University of Athens, 75 Iera Odos Str., 11855 Athens, Greece.
Plants (Basel). 2021 May 13;10(5):970. doi: 10.3390/plants10050970.
Grapevine bunch rot, caused by and , causes important economic losses every year in grape production. In the present study, we examined the plant protective activity of the biological control agents, K165, sp. FP12 and sp. FP15 against and on grapes. The in vitro experiments showed that strain K165 significantly reduced the growth of both fungi, while FP15 restricted the growth of and FP12 was ineffective. Following the in vitro experiments, we conducted in planta experiments on grape berries. It was shown that K165, FP12 and FP15 reduced rot severity by 81%, 57% and 37%, respectively, compared to the control, whereas, in the case of , the only protective treatment was that with K165, which reduced rot by 75%. The transcriptomic analysis of the genes encoding the pathogenesis-related proteins PR2, PR3, PR4 and PR5 indicates the activation of multiple defense responses involved in the biocontrol activity of the examined biocontrol agents.
由[未提及的病原菌]引起的葡萄串腐病每年都会给葡萄生产造成重大经济损失。在本研究中，我们检测了生防菌K165、[未提及的菌种]FP12和[未提及的菌种]FP15对葡萄上[未提及的病原菌]和[未提及的病原菌]的植物保护活性。体外实验表明，K165菌株显著抑制了两种真菌的生长，而FP15抑制了[未提及的病原菌]的生长，FP12则无效。体外实验之后，我们在葡萄浆果上进行了体内实验。结果表明，与对照相比，K165、FP12和FP15分别使[未提及的病原菌]引起的腐烂严重程度降低了81%、57%和37%，而对于[未提及的病原菌]，唯一有效的防治处理是使用K165，其使腐烂程度降低了75%。对编码病程相关蛋白PR2、PR3、PR4和PR5的基因进行的转录组分析表明，多种防御反应被激活，这些反应参与了所检测生防菌的生物防治活性。
