Min Sangwon, Song Eun-Ah Christine, Oyelakin Akinsola, Gluck Christian, Smalley Kirsten, Romano Rose-Anne
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, New York, United States of America.
Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York, United States of America.
PLoS One. 2018 Feb 20;13(2):e0192775. doi: 10.1371/journal.pone.0192775. eCollection 2018.
A better understanding of the normal and diseased biology of salivary glands (SG) has been hampered, in part, due to difficulties in cultivating and maintaining salivary epithelial cells. Towards this end, we have generated a mouse salivary gland epithelial cell (mSGc) culture system that is well-suited for the molecular characterization of SG cells and their differentiation program. We demonstrate that mSGc can be maintained for multiple passages without a loss of proliferation potential, readily form 3D-spheroids and importantly express a panel of well-established salivary gland epithelial cell markers. Moreover, mSGc 3D-spheroids also exhibit functional maturation as evident by robust agonist-induced intracellular calcium signaling. Finally, transcriptomic characterization of mSGc by RNA-seq and hierarchical clustering analysis with adult organ RNA-seq datasets reveal that mSGc retain most of the molecular attributes of adult mouse salivary gland. This well-characterized mouse salivary gland cell line will fill a critical void in the field by offering a valuable resource to examine various mechanistic aspects of mouse salivary gland biology.
唾液腺（SG）正常和患病生物学的深入理解在一定程度上受到阻碍，这是由于培养和维持唾液腺上皮细胞存在困难。为此，我们建立了一种小鼠唾液腺上皮细胞（mSGc）培养系统，该系统非常适合对唾液腺细胞及其分化程序进行分子特征分析。我们证明，mSGc可以传代多次而不丧失增殖潜力，易于形成三维球体，并且重要的是表达一组成熟的唾液腺上皮细胞标志物。此外，mSGc三维球体还表现出功能成熟，强大的激动剂诱导的细胞内钙信号就是明显的证据。最后，通过RNA测序对mSGc进行转录组特征分析，并与成年器官RNA测序数据集进行层次聚类分析，结果表明mSGc保留了成年小鼠唾液腺的大部分分子特征。这种特征明确的小鼠唾液腺细胞系将为研究小鼠唾液腺生物学的各种机制提供宝贵资源，填补该领域的一个关键空白。
