Graham L M, Brothers T E, Vincent C K, Burkel W E, Stanley J C
Department of Surgery, Case Western Reserve University, Cleveland, OH.
J Biomed Mater Res. 1991 Apr;25(4):525-33. doi: 10.1002/jbm.820250409.
The effect of an endothelial cell (EC) lining on intimal hyperplasia at the distal anastomosis of Dacron grafts was assessed in a canine model. Enzymatically derived autologous EC were used to seed 14 to 17 cm long, 4 mm I.D., knitted Dacron aortoiliac grafts implanted in an end-to-side manner in six dogs (Group I). Unseeded grafts were similarly implanted in six control dogs (Group II). All animals received acetylsalicylic acid (325 mg po qd) 24 h prior to graft placement and for 2 weeks postoperatively. Distal anastomotic intimal hyperplasia (AIH) and luminal surface EC coverage were quantitated at the conclusion of a 16-week study period. Patency for Group I and Group II grafts were 90% and 55%, respectively (p = 0.07). Maximum AIH, defined as the maximum reduction of luminal cross-sectional area at the distal anastomosis, was not significantly different between Group I (13.1 +/- 8.0%) and Group II (15.1 +/- 7.3%) conduits. However, AIH was inversely related to the extent of luminal EC coverage (r = -0.6, p less than 0.05), thus greater endothelialization was associated with decreased AIH. These data support the idea that EC coverage of the luminal surface of prosthetic vascular grafts may limit the development of AIH.
在犬类模型中评估了内皮细胞（EC）内衬对涤纶移植物远端吻合口内膜增生的影响。使用酶促获得的自体EC接种到14至17厘米长、内径4毫米的针织涤纶主-髂动脉移植物上，以端侧方式植入6只犬（I组）。未接种的移植物以类似方式植入6只对照犬（II组）。所有动物在植入移植物前24小时接受阿司匹林（325毫克口服，每日一次），并在术后持续2周。在为期16周的研究期结束时，对远端吻合口内膜增生（AIH）和管腔表面EC覆盖情况进行定量分析。I组和II组移植物的通畅率分别为90%和55%（p = 0.07）。I组（13.1 +/- 8.0%）和II组（15.1 +/- 7.3%）导管之间，定义为远端吻合口管腔横截面积最大减少量的最大AIH无显著差异。然而，AIH与管腔EC覆盖范围呈负相关（r = -0.6，p < 0.05），因此内皮化程度越高，AIH越低。这些数据支持以下观点：人工血管移植物管腔表面的EC覆盖可能会限制AIH的发展。
