Casu Rosanne E, Jarmey Janine M, Bonnett Graham D, Manners John M
CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Rd., St. Lucia, Queensland, 4067, Australia.
Funct Integr Genomics. 2007 Apr;7(2):153-67. doi: 10.1007/s10142-006-0038-z. Epub 2006 Nov 18.
Sugarcane is an important crop in tropical regions of the world, producing a very large biomass and accumulating large amounts of sucrose in the stem. In this study, we present the first report of transcript profiling using the GeneChip Sugarcane Genome Array. We have identified transcripts that are differentially expressed in the sugarcane stem during development by expression profiling using the array and total RNA derived from three disparate stem tissues (meristem, internodes 1-3, 8, and 20) from four replicates of the sugarcane variety Q117 grown in the field. We have identified 119 transcripts that were highly differentially expressed with development and have characterised members of the cellulose synthase (CesA) and cellulose synthase-like (Csl) gene families, which displayed coordinated expression during stem development. In addition, we determined that many other transcripts involved in cell wall metabolism and lignification were also co-expressed with members of the CesA and Csl gene families, offering additional insights into the dynamics of primary and secondary cell wall synthesis in the developing sugarcane stem.
甘蔗是世界热带地区的一种重要作物，能产生非常大量的生物量，并在茎中积累大量蔗糖。在本研究中，我们首次报道了使用基因芯片甘蔗基因组阵列进行转录谱分析。我们通过使用该阵列和来自田间种植的甘蔗品种Q117四个重复样本的三个不同茎组织（分生组织、第1 - 3节、第8节和第20节）的总RNA进行表达谱分析，鉴定了甘蔗茎在发育过程中差异表达的转录本。我们鉴定出119个在发育过程中高度差异表达的转录本，并对纤维素合酶（CesA）和类纤维素合酶（Csl）基因家族的成员进行了表征，这些成员在茎发育过程中表现出协同表达。此外，我们确定许多其他参与细胞壁代谢和木质化的转录本也与CesA和Csl基因家族的成员共表达，这为发育中的甘蔗茎中初生和次生细胞壁合成的动态过程提供了更多见解。
