Baldessari Fabio, Santiago Juan G
Department of Mechanical Engineering, Stanford University, Stanford, CA, USA.
J Nanobiotechnology. 2006 Nov 20;4:12. doi: 10.1186/1477-3155-4-12.
The relevant physical phenomena that dominate electrophoretic transport of ions and macromolecules within long, thin nanochannels are reviewed, and a few papers relevant to the discussion are cited. Sample ion transport through nanochannels is largely a function of their interaction with electric double layer. For small ions, this coupling includes the net effect of the external applied field, the internal field of the double layer, and the non-uniform velocity of the liquid. Adsorption/desorption kinetics and the effects of surface roughness may also be important in nanochannel electrophoresis. For macromolecules, the resulting motion is more complex as there is further coupling via steric interactions and perhaps polarization effects. These complex interactions and coupled physics represent a valuable opportunity for novel electrophoretic and chromatographic separations.
本文综述了在长而细的纳米通道中主导离子和大分子电泳输运的相关物理现象，并引用了一些与该讨论相关的论文。样品离子通过纳米通道的输运很大程度上取决于其与双电层的相互作用。对于小离子，这种耦合包括外部施加电场、双电层内部电场以及液体非均匀速度的净效应。吸附/解吸动力学和表面粗糙度的影响在纳米通道电泳中也可能很重要。对于大分子，由于存在空间相互作用以及可能的极化效应导致的进一步耦合，其运动更为复杂。这些复杂的相互作用和耦合物理现象为新型电泳和色谱分离提供了宝贵的机会。
