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蜕皮甾酮降低了果蝇细胞系中反转录转座子1731和412的转录水平。

Ecdysterone decreases the transcription level of the retrotransposons 1731 and 412 in a Drosophila cell line.

作者信息

Becker J, Micard D, Becker J L, Fourcade-Peronnet F, Dastugue B, Best-Belpomme M

机构信息

U-A CNRS 1135, Université P. et M. Curie, Paris, France.

出版信息

Cell Mol Biol. 1991;37(1):41-9.

PMID:1711928
Abstract

Cultured Drosophila melanogaster cells are responsive to the steroid hormone 20-hydroxyecdysone (ecdysterone). The transcripts of two copia-like transposable elements, 412 and 1731 were examined in cells treated for different lengths of time by the hormone. Using dot-blot and Northern-blot analysis, we have shown that the transcripts of these two retrotransposons were rapidly decreased by ecdysterone treatment.

摘要

培养的黑腹果蝇细胞对类固醇激素20-羟基蜕皮激素(蜕皮甾酮)有反应。在经该激素处理不同时长的细胞中,检测了两个类copia转座元件412和1731的转录本。通过斑点印迹和Northern印迹分析,我们发现这两个逆转座子的转录本在蜕皮激素处理后迅速减少。

相似文献

1
Ecdysterone decreases the transcription level of the retrotransposons 1731 and 412 in a Drosophila cell line.蜕皮甾酮降低了果蝇细胞系中反转录转座子1731和412的转录水平。
Cell Mol Biol. 1991;37(1):41-9.
2
Environmental stresses and mobilization of transposable elements in inbred lines of Drosophila melanogaster.黑腹果蝇近交系中的环境压力与转座元件的激活
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Dev Biol. 2002 Apr 1;244(1):170-9. doi: 10.1006/dbio.2002.0594.
4
Characterization and purification of DNA-RNA complexes related with 1731 and copia-like transposable elements in a Drosophila cell line.果蝇细胞系中与1731及类copia转座元件相关的DNA-RNA复合物的表征与纯化
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Maintenance of the Drosophila melanogaster genomic fragment harbouring the mdg4 copia-like retrotransposon in tobacco cells.
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Molecular analysis of the ecdysterone-inducible 2B5 "early' puff in Drosophila melanogaster.黑腹果蝇中蜕皮甾酮诱导的2B5“早期”胀泡的分子分析。
EMBO J. 1986 Jan;5(1):143-50. doi: 10.1002/j.1460-2075.1986.tb04188.x.
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Sequences involved in temperature and ecdysterone-induced transcription are located in separate regions of a Drosophila melanogaster heat shock gene.参与温度和蜕皮激素诱导转录的序列位于黑腹果蝇热休克基因的不同区域。
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Identification of a strong transcriptional activator for the copia retrotransposon responsible for its differential expression in Drosophila hydei and melanogaster cell lines.鉴定一种负责copia逆转座子在海德氏果蝇和黑腹果蝇细胞系中差异表达的强转录激活因子。
Biochem Biophys Res Commun. 1994 Aug 30;203(1):392-9. doi: 10.1006/bbrc.1994.2195.

引用本文的文献

1
A temperature cline in copy number for 412 but not roo/B104 retrotransposons in populations of Drosophila simulans.在拟暗果蝇种群中,412反转录转座子存在拷贝数温度梯度变化,而roo/B104反转录转座子则没有。
Proc Biol Sci. 1998 Jul 7;265(1402):1161-5. doi: 10.1098/rspb.1998.0413.
2
The microinjected Drosophila melanogaster 1731 retrotransposon is activated after the midblastula stage of the amphibian Pleurodeles waltl development.显微注射的黑腹果蝇1731反转录转座子在有尾两栖动物疣螈发育的囊胚中期之后被激活。
Genetica. 1994;92(2):107-14. doi: 10.1007/BF00163759.
3
A short 5' region of the long terminal repeat is required for regulation by hormone and heat shock of Drosophila retrotransposon 1731.
果蝇反转录转座子1731受激素和热休克调控需要长末端重复序列的一个短的5'区域。
Nucleic Acids Res. 1991 Oct 25;19(20):5689-93. doi: 10.1093/nar/19.20.5689.