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用于跳甲(Phyllotreta nemorum)中R基因的扩增片段长度多态性(AFLP)标记,该基因赋予对普通山芥防御的抗性。

AFLP markers for the R-gene in the flea beetle, Phyllotreta nemorum, conferring resistance to defenses in Barbarea vulgaris.

作者信息

Breuker Casper J, Victoir Kathleen, De Jong Peter W, van der Meijden Eddy, Brakefield Paul M, Vrieling Klaas

机构信息

Section of Evolutionary Biology, Institute of Biology, Leiden University, P.O. Box 9516, 2300 RA Leiden, The Netherlands.

出版信息

J Insect Sci. 2005 Dec 6;5:38. doi: 10.1093/jis/5.1.38.

DOI:10.1093/jis/5.1.38
PMID:17119620
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1615245/
Abstract

A so-called R-gene renders the yellow-striped flea beetle Phyllotreta nemorum L. (Coleoptera: Chrysomelidae: Alticinae) resistant to the defenses of the yellow rocket Barbarea vulgaris R.Br. (Brassicacea) and enables it to use it as a host plant in Denmark. In this study, genetic markers for an autosomal R-gene, inherited as a single, dominant locus in flea beetles from the Danish locality "Kvaerkeby" are described, and a genetic linkage map around this particular R-gene is constructed, using the technique of AFLP (Amplified Fragment Length Polymorphism).

摘要

一种所谓的R基因使黄条跳甲Phyllotreta nemorum L.(鞘翅目:叶甲科:跳甲亚科)对黄火箭Barbarea vulgaris R.Br.(十字花科)的防御机制产生抗性,并使其能够在丹麦将其用作寄主植物。在本研究中,描述了一个常染色体R基因的遗传标记,该基因在来自丹麦“Kvaerkeby”地区的跳甲中作为单一显性位点遗传,并利用扩增片段长度多态性(AFLP)技术构建了围绕该特定R基因的遗传连锁图谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c055/1615245/c86fa066a20b/i1536-2442-5-38-1-f102.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c055/1615245/bcbb46b4b59a/i1536-2442-5-38-1-f101.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c055/1615245/c86fa066a20b/i1536-2442-5-38-1-f102.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c055/1615245/bcbb46b4b59a/i1536-2442-5-38-1-f101.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c055/1615245/c86fa066a20b/i1536-2442-5-38-1-f102.jpg

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