Center for Molecular Plant Physiology, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, DK-1871 Frederiksberg C, Denmark.
Plant Physiol. 2009 Dec;151(4):1977-90. doi: 10.1104/pp.109.136952. Epub 2009 Oct 9.
Winter cress (Barbarea vulgaris) is resistant to a range of insect species. Some B. vulgaris genotypes are resistant, whereas others are susceptible, to herbivory by flea beetle larvae (Phyllotreta nemorum). Metabolites involved in resistance to herbivory by flea beetles were identified using an ecometabolomic approach. An F2 population representing the whole range from full susceptibility to full resistance to flea beetle larvae was generated by a cross between a susceptible and a resistant B. vulgaris plant. This F2 offspring was evaluated with a bioassay measuring the ability of susceptible flea beetle larvae to survive on each plant. Metabolites that correlated negatively with larvae survival were identified through correlation, cluster, and principal component analyses. Two main clusters of metabolites that correlate negatively with larvae survival were identified. Principal component analysis grouped resistant and susceptible plants as well as correlated metabolites. Known saponins, such as hederagenin cellobioside and oleanolic acid cellobioside, as well as two other saponins correlated significantly with plant resistance. This study shows the potential of metabolomics to identify bioactive compounds involved in plant defense.
冬葵（Barbarea vulgaris）对多种昆虫具有抗性。一些 B. vulgaris 基因型对叶甲幼虫（Phyllotreta nemorum）的取食具有抗性，而另一些则易感。本研究采用生态代谢组学方法鉴定了与叶甲幼虫取食抗性相关的代谢物。通过对一个易感和一个抗性 B. vulgaris 植物进行杂交，产生了一个代表从完全易感到完全抗叶甲幼虫的整个范围的 F2 群体。通过测量易感叶甲幼虫在每株植物上存活能力的生物测定法，对该 F2 后代进行了评估。通过相关性、聚类和主成分分析鉴定了与幼虫存活率呈负相关的代谢物。确定了两个与幼虫存活率呈负相关的主要代谢物簇。主成分分析将抗性和易感植物以及相关代谢物进行了分组。已知的皂苷，如hederagenin cellobioside 和 oleanolic acid cellobioside，以及另外两种与植物抗性显著相关的皂苷，也被鉴定出来。本研究表明代谢组学在鉴定参与植物防御的生物活性化合物方面具有潜力。
