焦磷酸测序法快速鉴定鲍曼不动杆菌中碳青霉烯水解型OXA类β-内酰胺酶
Pyrosequencing for rapid identification of carbapenem-hydrolysing OXA-type beta-lactamases in Acinetobacter baumannii.
作者信息
Naas T, Poirel L, Nordmann P
机构信息
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, K.-Bicêtre, France.
出版信息
Clin Microbiol Infect. 2006 Dec;12(12):1236-40. doi: 10.1111/j.1469-0691.2006.01563.x.
Carbapenem-resistant Acinetobacter baumannii isolates producing carbapenem-hydrolysing oxacillinases are emerging worldwide. These enzymes are divided into four phylogenetic subgroups: OXA-23-like, OXA-51-like, OXA-24-like and OXA-58-like. A PCR-based approach linked to pyrosequencing analysis was developed to identify the genes for these beta-lactamases. Carbapenem-hydrolysing oxacillinases were rapidly and unambiguously identified in a collection of carbapenem-resistant clinical isolates of A. baumannii and Acinetobacter junii. Pyrosequencing may provide a rapid tool for identification of OXA variants, thus avoiding delays inherent in classical sequencing methods.
产碳青霉烯水解型氧青霉烯酶的耐碳青霉烯鲍曼不动杆菌分离株正在全球范围内出现。这些酶被分为四个系统发育亚组：OXA-23样、OXA-51样、OXA-24样和OXA-58样。开发了一种与焦磷酸测序分析相关的基于PCR的方法来鉴定这些β-内酰胺酶的基因。在一组耐碳青霉烯的鲍曼不动杆菌和琼氏不动杆菌临床分离株中快速且明确地鉴定出了碳青霉烯水解型氧青霉烯酶。焦磷酸测序可为OXA变体的鉴定提供一种快速工具，从而避免传统测序方法固有的延迟。
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