焦磷酸测序快速检测 aac(6')-Ib-cr 喹诺酮耐药基因。
Rapid detection of aac(6')-Ib-cr quinolone resistance gene by pyrosequencing.
机构信息
Laboratoire de Bactériologie-Virologie-Hygiène, CHU Robert Debré, Rue du Général Koenig, 51092 Reims cedex, France.
出版信息
J Clin Microbiol. 2010 Jan;48(1):286-9. doi: 10.1128/JCM.01498-09. Epub 2009 Nov 18.
Abstract
Pyrosequencing was used to rapidly detect aac(6')-Ib and aac(6')-Ib-cr genes. This plasmid-mediated quinolone resistance determinant is increasing in extended-spectrum beta-lactamase-producing Enterobacteriaceae. This method is faster and more cost-effective than the methods previously described. Sequences obtained with this pyrosequencing method showed 100% concordance with conventional sequencing.
摘要
焦磷酸测序法被用于快速检测 aac(6')-Ib 和 aac(6')-Ib-cr 基因。这种质粒介导的喹诺酮类耐药决定因子在产超广谱β-内酰胺酶的肠杆菌科中不断增加。与之前描述的方法相比，该方法更快且更具成本效益。使用这种焦磷酸测序方法获得的序列与传统测序方法具有 100%的一致性。
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