Jessop D S, Chowdrey H S, Biswas S, Lightman S L
Neuroendocrinology Unit, Westminster Hospital, London, UK.
Neuropeptides. 1991 Mar;18(3):165-70. doi: 10.1016/0143-4179(91)90109-v.
Adult rats treated neonatally with monosodium glutamate (MSG) exhibit lesions in the arcuate nucleus of the hypothalamus. Following MSG lesioning, dopamine content in median eminence/arcuate nucleus (ME/AN) tissue extracts declined by 60-70%. Substance P (SP) content as determined by radioimmunoassay was significantly decreased in the paraventricular nucleus (PVN) (531 +/- 30 pg, mean +/- SEM) compared to controls (871 +/- 110 pg) but was unchanged in ME/AN extracts. Substance K (SK) content decreased to 257 +/- 20 pg in the PVN of lesioned animals compared to controls (367 +/- 31 pg) and the ME/AN content of SK was also significantly decreased (236 +/- 36 pg compared to control levels of 619 +/- 65 pg). The CRF-41 content of the PVN and ME/AN was unchanged by MSG lesioning, indicating that these areas are not affected by MSG. The partial depletion of SP and SK in the PVN following MSG treatment provides evidence that at least some of the neurokinin content of the PVN may originate in cell bodies of the arcuate nucleus. However, the lack of response of ME/AN SP to MSG treatment may suggest that the arcuate nucleus is not the major source of SP in the median eminence.
新生期用谷氨酸单钠（MSG）处理的成年大鼠，其下丘脑弓状核出现损伤。MSG损伤后，正中隆起/弓状核（ME/AN）组织提取物中的多巴胺含量下降了60 - 70%。通过放射免疫测定法测定，与对照组（871 +/- 110 pg）相比，室旁核（PVN）中的P物质（SP）含量显著降低（531 +/- 30 pg，平均值 +/- 标准误），但ME/AN提取物中的含量未发生变化。与对照组（367 +/- 31 pg）相比，损伤动物PVN中的K物质（SK）含量降至257 +/- 20 pg，且ME/AN中SK的含量也显著降低（与对照组水平619 +/- 65 pg相比为236 +/- 36 pg）。MSG损伤未改变PVN和ME/AN中的CRF - 41含量，表明这些区域不受MSG影响。MSG处理后PVN中SP和SK的部分耗竭表明，PVN中至少部分神经激肽含量可能起源于弓状核的细胞体。然而，ME/AN中的SP对MSG处理无反应，这可能表明弓状核不是正中隆起中SP的主要来源。
