Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, Beijing, 100081, China.
Beijing Institute of Technology (Tangshan) Translational Research Center, Hebei, 063611, China.
Microb Cell Fact. 2023 Aug 5;22(1):147. doi: 10.1186/s12934-023-02144-9.
Vanillin (4-hydroxy-3-methoxybenzaldehyde) is one of the most popular flavors with wide applications in food, fragrance, and pharmaceutical industries. However, the high cost and limited yield of plant extraction failed to meet the vast market demand of natural vanillin. Vanillin biotechnology has emerged as a sustainable and cost-effective alternative to supply vanillin. In this review, we explored recent advances in vanillin biosynthesis and highlighted the potential of vanillin biotechnology. In particular, we addressed key challenges in using microorganisms and provided promising approaches for improving vanillin production with a special focus on chassis development, pathway construction and process optimization. Future directions of vanillin biosynthesis using inexpensive precursors are also thoroughly discussed.
香草醛（4-羟基-3-甲氧基苯甲醛）是最受欢迎的香料之一，广泛应用于食品、香料和制药行业。然而，植物提取的高成本和有限产量无法满足天然香草醛的巨大市场需求。香草醛生物技术作为一种可持续且具有成本效益的替代方法，已经出现。在本综述中，我们探讨了香草醛生物合成的最新进展，并强调了香草醛生物技术的潜力。特别是，我们解决了使用微生物的关键挑战，并提供了有前途的方法来改善香草醛的生产，特别关注底盘开发、途径构建和过程优化。还彻底讨论了使用廉价前体进行香草醛生物合成的未来方向。
