Roll D E, Murphy B J, Laderoute K R, Sutherland R M, Smith H C
Department of Chemistry, Roberts Wesleyan College, Rochester, NY 14624.
Mol Cell Biochem. 1991 May 15;103(2):141-8. doi: 10.1007/BF00227480.
Ischemic stress of cells within solid tumors arises from inadequate perfusion of regions of the tumor and results in microenvironments which are hypoxic and deficient in nutrient delivery and waste product removal. Stressed cells within these microenvironments show growth inhibition and synthesize unique sets of proteins referred to as glucose and oxygen regulated proteins (GRPs and ORPs respectively). The commonality of proteins induced by glucose-starvation and hypoxia has not been proven. To this end, ORPs were induced in Chinese hamster ovary cells in the presence of high glucose concentration in the media and ORP 80 isolated from two dimension gels. Eleven tryptic peptides of the 80 kDa ORP were sequenced and found to be identical to GRP 78 sequences. The data demonstrate that GRP 78 and ORP 80 have the same primary amino acid sequence and suggest that glucose-starvation and hypoxia can induce the same cellular responses.
实体肿瘤内细胞的缺血应激源于肿瘤区域灌注不足，导致微环境缺氧，营养物质供应不足且废物清除不畅。这些微环境中的应激细胞表现出生长抑制，并合成一组独特的蛋白质，分别称为葡萄糖调节蛋白（GRPs）和氧调节蛋白（ORPs）。葡萄糖饥饿和缺氧诱导产生的蛋白质的共性尚未得到证实。为此，在中国仓鼠卵巢细胞中，在培养基中高葡萄糖浓度存在的情况下诱导产生ORPs，并从二维凝胶中分离出ORP 80。对80 kDa的ORP的11个胰蛋白酶肽段进行了测序，发现与GRP 78序列相同。数据表明，GRP 78和ORP 80具有相同的一级氨基酸序列，并表明葡萄糖饥饿和缺氧可诱导相同的细胞反应。
