Sciandra J J, Subjeck J R, Hughes C S
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4843-7. doi: 10.1073/pnas.81.15.4843.
In this report we examine the effects of chronic anaerobic exposure and subsequent reoxygenation on protein synthesis patterns in Chinese hamster ovary cells. It is observed by two-dimensional gel electrophoresis (isoelectric focusing/NaDodSO4/PAGE) that the transition from an atmospheric environment to an anaerobic state transiently induces the major heat-shock proteins (at 68 and 89 kDa). As the period of anaerobiosis increases, this heat-shock induction disappears and a new set of proteins (at 76 and 97 kDa) is induced. By two-dimensional gel electrophoresis and partial proteolytic mapping, these new proteins, which are induced by anaerobic exposures exceeding 12 hr, are identical to 76 and 97 kDa (p76 and p97, respectively) proteins induced by extended periods of glucose deprivation (greater than 14 hr) when oxygen is present. Furthermore, the induction of these proteins under anoxia occurs in the presence of glucose, and increasing the glucose content of the starting media does not affect the induction. When anaerobic p76 and p97 induced cells are returned to atmospheric oxygen, p76 and p97 are repressed, while the heat-shock proteins are again transiently induced. This work further suggests the importance of deprivation and release environments in controlling the expression of these two stress protein systems. It is suggested that their natural expression may be determined by comparable circumstances.
在本报告中，我们研究了慢性厌氧暴露及随后的复氧对中国仓鼠卵巢细胞蛋白质合成模式的影响。通过二维凝胶电泳（等电聚焦/SDS/PAGE）观察到，从大气环境转变为厌氧状态会短暂诱导主要的热休克蛋白（68 kDa和89 kDa）。随着厌氧时间的增加，这种热休克诱导消失，同时诱导出一组新的蛋白质（76 kDa和97 kDa）。通过二维凝胶电泳和部分蛋白水解图谱分析，这些在超过12小时的厌氧暴露下诱导产生的新蛋白质，与在有氧存在时延长葡萄糖剥夺时间（超过14小时）所诱导的76 kDa和97 kDa（分别为p76和p97）蛋白质相同。此外，在缺氧条件下这些蛋白质的诱导发生在有葡萄糖存在时，并且增加起始培养基中的葡萄糖含量并不影响诱导。当厌氧诱导产生p76和p97的细胞恢复到大气氧环境时，p76和p97受到抑制，而热休克蛋白再次被短暂诱导。这项工作进一步表明了剥夺和释放环境在控制这两种应激蛋白系统表达中的重要性。有人提出它们的天然表达可能由类似的情况决定。
