Wang Y Q, Lü L, Weng S P, Huang J N, Chan S-M, He J G
State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan University, Guangzhou, P.R. China.
Arch Virol. 2007;152(4):763-73. doi: 10.1007/s00705-006-0870-4. Epub 2006 Nov 27.
Infectious spleen and kidney necrosis virus-like (ISKNV-like) virus causes a serious systemic disease with high morbidity and mortality of freshwater and marine fishes. Based on the ISKNV putative major capsid protein (MCP), the vascular endothelial growth factor (VEGF), the mRNA capping enzyme (Capping), and the tumor necrosis factor receptor-associated protein (TNFR) genes, primers were designed and used in PCR to determine the host range of ISKNV-like viruses. From the sampling of >1600 marine fishes representing 6 orders, 25 families, and 86 species collected in the South China Sea, 13 cultured fish species (141 fish) and 39 wild fish species (102 fish) were confirmed hosts of ISKNV-like viruses. The average percentage of infection of ISKNV-like viruses was 14.6%. The results from phylogenetic analysis of these genes revealed that ISKNV-like viruses could be placed into two clusters: cluster I was more related to ISKNV; cluster II included OSGIV (orange-spotted grouper iridovirus) and RBIV (rock bream iridovirus), and was quite different from ISKNV. The results of this study can contribute to the prediction and prevention of ISKNV disease outbreaks.
传染性脾肾坏死样病毒（ISKNV样病毒）可引发淡水和海水鱼类严重的全身性疾病，发病率和死亡率都很高。基于ISKNV推定的主要衣壳蛋白（MCP）、血管内皮生长因子（VEGF）、mRNA封端酶（Capping）和肿瘤坏死因子受体相关蛋白（TNFR）基因，设计了引物并用于PCR，以确定ISKNV样病毒的宿主范围。在对从南海采集的代表6目、25科和86种的1600多条海水鱼进行采样后，确认13种养殖鱼类（141条鱼）和39种野生鱼类（102条鱼）为ISKNV样病毒的宿主。ISKNV样病毒的平均感染率为14.6%。对这些基因的系统发育分析结果表明，ISKNV样病毒可分为两个簇：簇I与ISKNV关系更密切；簇II包括橙点石斑鱼虹彩病毒（OSGIV）和条石鲷虹彩病毒（RBIV），与ISKNV有很大不同。本研究结果有助于预测和预防ISKNV疾病的爆发。
