Lü Ling, Zhou Song Y, Chen Cheng, Weng Shao P, Chan Siu-Ming, He Jian G
State Key Laboratory for Biocontrol, School of Life Sciences, Zhongshan University, Guangzhou 510275, P. R. China.
Virology. 2005 Aug 15;339(1):81-100. doi: 10.1016/j.virol.2005.05.021.
Orange-spotted grouper iridovirus (OSGIV) was the causative agent of serious systemic diseases with high mortality in the cultured orange-spotted grouper, Epinephelus coioides. Here we report the complete genome sequence of OSGIV. The OSGIV genome consists of 112,636 bp with a G+C content of 54%. 121 putative open reading frames (ORF) were identified with coding capacities for polypeptides varying from 40 to 1168 amino acids. The majority of OSGIV shared homologies to other iridovirus genes. Phylogenetic analysis of the major capsid protein, ATPase, cytosine DNA methyl transferase and DNA polymerase indicated that OSGIV was closely related to infectious spleen and kidney necrosis virus (ISKNV) and rock bream iridovirus (RBIV), but differed from lymphocytisvirus and ranavirus. The determination of the genome of OSGIV will facilitate a better understanding of the molecular mechanism underlying the pathogenesis of the OSGIV and may provide useful information to develop diagnosis method and strategies to control outbreak of OSGIV.
橙点石斑鱼虹彩病毒（OSGIV）是养殖的棕点石斑鱼（Epinephelus coioides）中导致严重全身性疾病且死亡率很高的病原体。在此，我们报告了OSGIV的完整基因组序列。OSGIV基因组由112,636个碱基对组成，G+C含量为54%。共鉴定出121个推定的开放阅读框（ORF），其编码的多肽长度从40到1168个氨基酸不等。OSGIV的大多数基因与其他虹彩病毒基因具有同源性。对主要衣壳蛋白、ATP酶、胞嘧啶DNA甲基转移酶和DNA聚合酶的系统发育分析表明，OSGIV与传染性脾肾坏死病毒（ISKNV）和条石鲷虹彩病毒（RBIV）密切相关，但与淋巴细胞病毒和蛙病毒不同。OSGIV基因组的测定将有助于更好地理解OSGIV发病机制的分子机制，并可能为开发诊断方法和控制OSGIV爆发的策略提供有用信息。
