Gill Ron, Freeman Ronit, Xu Jian-Ping, Willner Itamar, Winograd Shira, Shweky Itzik, Banin Uri
Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
J Am Chem Soc. 2006 Dec 6;128(48):15376-7. doi: 10.1021/ja066636t.
CdSe/ZnS QDs enable the optical probing of the biocatalytic oxidation of tyrosine derivatives and of the scission of peptides by thrombin. CdSe/ZnS QDs were modified with tyrosine methyl ester or with a tyrosine-containing peptide. The tyrosine units were reacted with tyrosinase/O2 to yield the respective l-DOPA and quinone derivatives. The luminescence of QDs modified by the enzyme-generated quinone units is quenched. The quinone-functionalized peptide associated with the QDs was cleaved by thrombin, a process that restored the luminescence of the QDs.
CdSe/ZnS量子点能够对酪氨酸衍生物的生物催化氧化以及凝血酶对肽的裂解进行光学探测。CdSe/ZnS量子点用酪氨酸甲酯或含酪氨酸的肽进行了修饰。酪氨酸单元与酪氨酸酶/O₂反应生成相应的左旋多巴和醌衍生物。由酶生成的醌单元修饰的量子点的发光被淬灭。与量子点相关的醌功能化肽被凝血酶裂解，这一过程恢复了量子点的发光。
