Dallman M J, Larsen C P, Morris P J
Nuffield Department of Surgery, John Radcliffe Hospital, Headington, Oxford, United Kingdom.
J Exp Med. 1991 Aug 1;174(2):493-6. doi: 10.1084/jem.174.2.493.
Cytokine gene transcription has been analyzed by direct analysis of RNA obtained from mouse heterotopic cardiac transplants. The level of expression of the cytokine genes was assessed using semiquantitative polymerase chain reaction (PCR). Expression of the cytokines investigated fell into three groups. The first group included interleukin 1 beta (IL-1 beta), IL-5, IL-6, and interferon gamma (IFN gamma). These genes were expressed in normal heart tissue at low level and were upregulated following both syngeneic and allogeneic transplantation. Genes in the second group (IL-1 alpha, IL-3) were not expressed at detectable levels in normal heart but were induced following either syngeneic or allogeneic heart grafting. IL-2, IL-4, and tumor necrosis factor beta (IFN beta) comprised the third group and these cytokines were expressed only in allogeneic grafts after transplantation.
通过对从小鼠异位心脏移植中获取的RNA进行直接分析，对细胞因子基因转录进行了研究。使用半定量聚合酶链反应（PCR）评估细胞因子基因的表达水平。所研究的细胞因子的表达分为三组。第一组包括白细胞介素1β（IL-1β）、IL-5、IL-6和干扰素γ（IFNγ）。这些基因在正常心脏组织中低水平表达，在同基因和异基因移植后均上调。第二组基因（IL-1α、IL-3）在正常心脏中未检测到表达，但在同基因或异基因心脏移植后被诱导表达。IL-2、IL-4和肿瘤坏死因子β（IFNβ）组成第三组，这些细胞因子仅在移植后的异基因移植物中表达。
