Llorente L, Crevon M C, Karray S, Defrance T, Banchereau J, Galanaud P
INSERM U131, Clamart, France.
Eur J Immunol. 1989 Apr;19(4):765-9. doi: 10.1002/eji.1830190429.
We tested the effect of interleukin (IL) 4 on the specific IgM antibody response induced by trinitrophenylated-polyacrylamide beads (TNP-PAA) in cultures of human B cells. T cell help was provided by exogeneous IL2. IL4 profoundly suppressed the response to optimal concentrations (50 U/ml) of IL2, with a 50% inhibitory concentration of 6 U/ml. This was due neither to a shift in the kinetics nor to a switch to an IgG response. The production of anti-TNP antibody (as measured by an enzyme-linked immunosorbent assay in the culture supernatant) was inhibited to the same extent as the generation of plaque-forming cells. The effect of IL4 was completely abolished by a neutralizing antibody toward IL4. Kinetic studies showed that IL4 had to be present during the first 48 h of culture to fully inhibit the response. The sequential stimulation of B cells by antigen and by IL2 showed that IL4 does not negatively interfere with signaling through membrane Ig but counteracts the effect of IL2 on antigen-activated B cells.
我们检测了白细胞介素（IL）4对人B细胞培养物中三硝基苯化聚丙烯酰胺珠（TNP-PAA）诱导的特异性IgM抗体反应的影响。外源性IL2提供T细胞辅助。IL4显著抑制对最佳浓度（50 U/ml）IL2的反应，50%抑制浓度为6 U/ml。这既不是由于动力学改变，也不是由于转向IgG反应。抗TNP抗体的产生（通过培养上清液中的酶联免疫吸附测定法测量）与噬斑形成细胞的产生受到同等程度的抑制。针对IL4的中和抗体完全消除了IL4的作用。动力学研究表明，IL4必须在培养的前48小时存在才能完全抑制反应。抗原和IL2对B细胞的顺序刺激表明，IL4不会对通过膜免疫球蛋白的信号传导产生负面干扰，但会抵消IL2对抗原激活的B细胞的作用。
