ERF/AP2结构域中保守的丙氨酸37对于与DRE元件和GCC盒结合至关重要。
The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC box.
作者信息
Liu Yang, Zhao Tong-Jin, Liu Jing-Mei, Liu Wei-Qun, Liu Qiang, Yan Yong-Bin, Zhou Hai-Meng
机构信息
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
出版信息
FEBS Lett. 2006 Feb 20;580(5):1303-8. doi: 10.1016/j.febslet.2006.01.048. Epub 2006 Jan 26.
Four AP2/EREBP genes encoding putative ethylene-responsive element binding factor (ERF)/AP2 domains were cloned from Brassica napus, and these genes could be induced by low temperature, ethylene, drought, high salinity, abscisic acid and jasmonate treatments. These four genes, named BnDREBIII-1 to BnDREBIII-4, were highly homologous and the 37th amino acid was the only difference among their ERF/AP2 domains. BnDREBIII-1 was demonstrated to be able to bind to both dehydration-responsive element and the GCC box and transactivate the expression of downstream genes, while BnDREBIII-4 could bind neither. Further results suggested that Ala37 might play a crucial role in the DNA binding or the stability of the ERF/AP2 domain.
从甘蓝型油菜中克隆出了四个编码假定乙烯反应元件结合因子（ERF）/AP2结构域的AP2/EREBP基因，这些基因可被低温、乙烯、干旱、高盐、脱落酸和茉莉酸处理诱导。这四个基因命名为BnDREBIII-1至BnDREBIII-4，高度同源，其ERF/AP2结构域中第37位氨基酸是唯一的差异。结果表明，BnDREBIII-1能够与脱水反应元件和GCC盒结合，并激活下游基因的表达，而BnDREBIII-4两者均不能结合。进一步的结果表明，丙氨酸37可能在ERF/AP2结构域的DNA结合或稳定性中起关键作用。
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