Fu Xiao-Yan, Zhang Zhen, Peng Ri-He, Xiong Ai-Sheng, Liu Jin-Ge, Wu Li-Juan, Gao Feng, Zhu Hong, Guo Zhao-Kui, Yao Quan-Hong
Department of Horticulture, Nanjing Agricultural University, Weigang, Nanjing, 210095, P.R. China.
Biotechnol Lett. 2007 Aug;29(8):1293-9. doi: 10.1007/s10529-007-9370-1. Epub 2007 May 22.
Using a yeast one-hybrid method, a transcription factor, OsAP25, which interacts specifically with a GCC box was isolated from rice. The OsAP25 protein contained a conserved ethylene-responsive element binding factor (ERF) domain which shared identity with other reported ERF domains. Phylogenetic analysis showed that OsAP25 could be categorized into class III ERF of the previously characterized ERF proteins on an evolutionary relationship. The semi-quantitative RT-PCR analysis revealed that OsAP25 gene was constitutively expressed in leaves, roots, growing points, flower, bolting stage and grain filling stage. In addition, OsAP25 gene was induced by NaCl, cold, drought, abscisic acid and exogenous ethylene.
利用酵母单杂交方法，从水稻中分离出一种与GCC盒特异性相互作用的转录因子OsAP25。OsAP25蛋白含有一个保守的乙烯反应元件结合因子（ERF）结构域，该结构域与其他已报道的ERF结构域具有同源性。系统发育分析表明，基于进化关系，OsAP25可归类为先前鉴定的ERF蛋白中的III类ERF。半定量RT-PCR分析显示，OsAP25基因在叶片、根、生长点、花、抽穗期和灌浆期组成型表达。此外，OsAP25基因受NaCl、低温、干旱、脱落酸和外源乙烯诱导。
