Moubayed Noemi, Weichenthal Michael, Harder Jürgen, Wandel Elke, Sticherling Michael, Gläser Regine
Department of Dermatology, Allergology and Venerology, University Hospital of Schleswig-Holstein, Campus Kiel, Schittenhelmstr. 7, 24105 Kiel, Germany.
J Cancer Res Clin Oncol. 2007 Apr;133(4):253-61. doi: 10.1007/s00432-006-0164-y. Epub 2006 Nov 29.
Psoriasin (S100A7) has originally been described to be expressed by psoriatic keratinocytes possibly as a result of altered differentiation and inflammation. As psoriasin was found to be overexpressed in human breast and bladder cancer suggesting a role in tumour progression, we investigated the expression of psoriasin in human epithelial skin tumours.
Realtime reverse transcription-polymerase chain reaction experiments were performed to analyse the mRNA-expression levels of psoriasin together with involucrin as a marker for epithelial differentiation and interleukin-8 (IL-8) as a marker for inflammation in skin biopsy samples from patients with precancerous skin lesions (PSL, n = 6), squamous cell carcinoma (SCC, n = 11), basal cell carcinoma (BCC, n = 17), and healthy controls (n = 10).
Unexpectedly, mRNA expression levels for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) revealed a variation of up to 600-fold in all cDNA-samples under investigation, indicating that GAPDH is not suitable as a housekeeping gene in human skin samples. Psoriasin mRNA expression was significantly up-regulated in samples of PSL, SCC and BCC. In situ hybridisation and immunohistochemical examinations identified psoriasin mRNA and protein expression in the differentiated layers of the epidermis. IL-8 mRNA expression was significantly up-regulated in SCC, however, there was no correlation between elevated levels of psoriasin and the expression of IL-8.
Similar to the findings in breast and bladder cancer, the up-regulation of psoriasin might play a role in the progression of skin cancer. The expression of psoriasin in human skin tumours seems to be independent from differentiation and inflammation.
最初有研究表明，牛皮癣素（S100A7）可能由于分化改变和炎症而在银屑病角质形成细胞中表达。由于发现牛皮癣素在人类乳腺癌和膀胱癌中过度表达，提示其在肿瘤进展中发挥作用，因此我们研究了牛皮癣素在人类上皮性皮肤肿瘤中的表达。
进行实时逆转录 - 聚合酶链反应实验，以分析牛皮癣素的mRNA表达水平，同时分析作为上皮分化标志物的内披蛋白以及作为皮肤活检样本中炎症标志物的白细胞介素 - 8（IL - 8）的表达水平。这些皮肤活检样本来自癌前皮肤病变患者（PSL，n = 6）、鳞状细胞癌患者（SCC，n = 11）、基底细胞癌患者（BCC，n = 17）以及健康对照者（n = 10）。
出乎意料的是，在所研究的所有cDNA样本中，甘油醛 - 3 - 磷酸脱氢酶（GAPDH）的mRNA表达水平变化高达600倍，这表明GAPDH不适宜作为人类皮肤样本中的管家基因。牛皮癣素mRNA表达在PSL、SCC和BCC样本中显著上调。原位杂交和免疫组化检查确定了牛皮癣素mRNA和蛋白在表皮分化层中的表达。IL - 8 mRNA表达在SCC中显著上调，然而，牛皮癣素水平升高与IL - 8表达之间没有相关性。
与乳腺癌和膀胱癌的研究结果相似，牛皮癣素的上调可能在皮肤癌进展中起作用。牛皮癣素在人类皮肤肿瘤中的表达似乎与分化和炎症无关。
