霍乱弧菌O1小川血清型特异性相关基因的鉴定及核苷酸序列测定
Identification and nucleotide sequence determination of the gene responsible for Ogawa serotype specificity of V. cholerae 01.
作者信息
Ito T, Ohshita Y, Hiramatsu K, Yokota T
机构信息
Department of Bacteriology, Faculty of Medicine, Juntendo University, Tokyo, Japan.
出版信息
FEBS Lett. 1991 Jul 29;286(1-2):159-62. doi: 10.1016/0014-5793(91)80964-5.
The gene encoding a protein of 27 kDa, which is specifically expressed in Vibrio cholerae of serotype Ogawa, was identified and its nucleotide sequence determined. The plasmid carrying this gene was found to convert serotype specificity from Inaba to Ogawa when introduced into the Escherichia coli DH5(pVCI112) cell which harbors a cloned 20-kilobase genomic DNA fragment of V. cholerae NIH35A3 and expresses the 01 antigen of Inaba serotype.
编码一种27 kDa蛋白的基因在小川血清型霍乱弧菌中特异性表达，该基因已被鉴定并测定了其核苷酸序列。当携带该基因的质粒被导入大肠杆菌DH5(pVCI112)细胞时，发现它能将血清型特异性从稻叶型转变为小川型，该大肠杆菌细胞含有霍乱弧菌NIH35A3克隆的20千碱基基因组DNA片段，并表达稻叶血清型的01抗原。
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