霍乱弧菌O1血清型特异性决定基因组区域的鉴定

Identification of the genomic region determining serotype specificity of Vibrio cholerae 01.

作者信息

Ito T, Ohshita Y, Nakamoto T, Hiramatsu K, Yokota T

机构信息

Department of Bacteriolog, Faculty of Medicine, Juntendo University, Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Mar 15;175(2):679-84. doi: 10.1016/0006-291x(91)91619-n.

DOI:10.1016/0006-291x(91)91619-n

PMID:2018510

Abstract

A 2.1-kb genomic region responsible for Ogawa serotype specificity of Vibrio cholerae 01 was identified by cosmid cloning and recombinant plasmid experiments. The plasmid carrying this region derived from Ogawa type Vibrio cholerae NIH 41 coded for a specific protein of 27 kD, and was found to convert serotype specificity from Inaba to Ogawa when co-introduced into the Escherichia coli cells harboring a cloned 20-kilobase genomic DNA fragment of Inaba type Vibrio cholerae 35A3.

摘要

通过黏粒克隆和重组质粒实验，鉴定出一个负责霍乱弧菌O1小川血清型特异性的2.1kb基因组区域。携带该区域的质粒源自小川型霍乱弧菌NIH 41，编码一种27kD的特异性蛋白质，当与携带稻叶型霍乱弧菌35A3的20kb基因组DNA片段克隆体共同导入大肠杆菌细胞时，发现它可将血清型特异性从稻叶型转变为小川型。