Hisatsune K, Kondo S, Iguchi T, Ito T, Hiramatsu K
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
Microbiol Immunol. 1996;40(9):621-6. doi: 10.1111/j.1348-0421.1996.tb01119.x.
Structural and serological studies were performed with the lipopolysaccharide (LPS) expressed by Escherichia coli K12 strains No. 30 and No. 64, into which cosmid clones derived from Vibrio cholerae O1 NIH 41 (Ogawa) and NIH 35A3 (Inaba) had been introduced, respectively. The two recombinant strains, No. 30 (Ogawa) and No. 64 (Inaba), produced LPS that included, in common, the O-polysaccharide chain composed of an alpha (1-->2)-linked N-(3-deoxy-L-glycero-tetronyl)-D-perosamine (4-amino-4,6-dideoxy-D-manno-pyranose) homopolymer attached to the core oligosaccharide of the LPS of E. coli K12. Structural analysis revealed the presence of N-(3-deoxy-L-glycero-tetronyl)-2-O-methyl-D-perosamine at the non-reducing terminus of the O-polysaccharide chain of LPS from No. 30 (Ogawa) but not from No. 64 (Inaba). Serological analysis revealed that No. 30 (Ogawa) and No. 64 (Inaba) LPS were found to share the group antigen factor A of V. cholerae O1. They were distinguished by presence of the Ogawa antigen factor B [co-existing with relatively small amounts of the Inaba antigen factor (c)] in the former LPS and the Inaba antigen factor C in the latter LPS. It appears, therefore, that No. 30 (Ogawa) and No. 64 (Inaba) have O-antigenic structures that are fully consistent with the AB(c) structure for the Ogawa and the AC structure for the Inaba O-forms of V. cholerae O1, respectively. Thus, the present study clearly confirmed our previous finding that the Ogawa antigenic factor B is substantially related to the 2-O-methyl group at the non-reducing terminus of the alpha (1-->2)-linked N-(3-deoxy-L-glycero-tetronyl)-D-perosamine homopolymer that forms the O-polysaccharide chain of LPS of V. cholerae O1 (Ogawa).
对大肠杆菌K12 30号菌株和64号菌株所表达的脂多糖（LPS）进行了结构和血清学研究，这两种菌株分别导入了源自霍乱弧菌O1 NIH 41（小川型）和NIH 35A3（稻叶型）的黏粒克隆。这两种重组菌株，30号（小川型）和64号（稻叶型），产生的LPS共同包含由α（1→2）连接的N-（3-脱氧-L-甘油四糖基）-D-过氧胺（4-氨基-4,6-二脱氧-D-甘露吡喃糖）同聚物组成的O-多糖链，该同聚物连接到大肠杆菌K12 LPS的核心寡糖上。结构分析显示，30号（小川型）LPS的O-多糖链非还原末端存在N-（3-脱氧-L-甘油四糖基）-2-O-甲基-D-过氧胺，而64号（稻叶型）则没有。血清学分析显示，30号（小川型）和64号（稻叶型）LPS具有霍乱弧菌O1的群抗原因子A。它们的区别在于，前一种LPS中存在小川抗原因子B [与相对少量的稻叶抗原因子（c）共存]，而后一种LPS中存在稻叶抗原因子C。因此，30号（小川型）和64号（稻叶型）似乎分别具有与霍乱弧菌O1小川型的AB（c）结构和稻叶型的AC结构完全一致的O-抗原结构。因此，本研究清楚地证实了我们之前的发现，即小川抗原因子B与形成霍乱弧菌O1（小川型）LPS的O-多糖链的α（1→2）连接的N-（3-脱氧-L-甘油四糖基）-D-过氧胺同聚物非还原末端的2-O-甲基基团密切相关。
