Ward H M, Manning P A
Department of Microbiology and Immunology, University of Adelaide, Australia.
Mol Gen Genet. 1989 Aug;218(2):367-70. doi: 10.1007/BF00331294.
Vibrio cholerae strains of the 01 serotype have been classified into three subclasses, Ogawa, Inaba and Hikojima, which are associated with the O-antigen of the lipopolysaccharide (LPS). The DNA encoding the biosynthesis of the O-antigen, the rfb locus, has been cloned and analysed (Manning et al. 1986; Ward et al. 1987). Transposon mutagenesis of the Inaba and Ogawa strains of V. cholerae, using Tn5 or Tn2680 allowed the isolation of a series of independent mutants in each of these serotypes. Some of the insertions were mapped to the rfb region by Southern hybridization using the cloned rfb DNA as a probe, confirming this location to be responsible for both O-antigen production and serotype specificity. The other insertions allowed a second region to be identified which is involved in V. cholerae LPS biosynthesis.
01血清型霍乱弧菌菌株已被分为三个亚类，即小川型、稻叶型和彦岛型，它们与脂多糖（LPS）的O抗原相关。编码O抗原生物合成的DNA，即rfb基因座，已被克隆和分析（曼宁等人，1986年；沃德等人，1987年）。利用Tn5或Tn2680对霍乱弧菌的稻叶型和小川型菌株进行转座子诱变，使得在每种血清型中都分离出了一系列独立的突变体。通过使用克隆的rfb DNA作为探针进行Southern杂交，将一些插入片段定位到rfb区域，证实该位置负责O抗原的产生和血清型特异性。其他插入片段使得能够鉴定出参与霍乱弧菌LPS生物合成的第二个区域。
