Young E G, Hanson M R
Cell. 1987 Jul 3;50(1):41-9. doi: 10.1016/0092-8674(87)90660-x.
Sequencing of an open reading frame associated with cytoplasmic male sterility (CMS) in Petunia has revealed a gene fusion (the Pcf gene) containing the 5'-flanking and amino-terminal transmembrane segment of the ATP synthase proteolipid gene (atp9), parts of the cytochrome oxidase subunit II (coxII) coding region, and the carboxyl terminus and 3'-flanking region of an unidentified reading frame (urfS). The coxII region has several small deletions and tandem repeats that remove all of the segments coding for the residues involved in copper binding, but may possibly maintain the cytochrome c binding site. Normal atp9 and coxII genes and their transcripts are also present in the sterile cytoplasm. S1 nuclease protection studies identify fused gene transcripts only in CMS lines, with an increase in transcript amount in anthers relative to leaves.
矮牵牛中与细胞质雄性不育（CMS）相关的一个开放阅读框的测序揭示了一个基因融合体（Pcf基因），它包含ATP合酶蛋白脂质基因（atp9）的5'侧翼和氨基末端跨膜区段、细胞色素氧化酶亚基II（coxII）编码区的部分序列，以及一个未鉴定阅读框（urfS）的羧基末端和3'侧翼区。coxII区域有几个小的缺失和串联重复，这些缺失和重复去除了所有编码参与铜结合的残基的区段，但可能保留了细胞色素c结合位点。正常的atp9和coxII基因及其转录本也存在于不育细胞质中。S1核酸酶保护研究仅在CMS系中鉴定出融合基因转录本，花药中的转录本量相对于叶片有所增加。
