Oliver Roly, May Eric, Williams John
Department of Civil Engineering, University of Portsmouth, Lion Gate Building, Lion Terrace, Portsmouth, Hants, PO1 3HF United Kingdom.
Sci Total Environ. 2007 Jan 1;372(2-3):605-14. doi: 10.1016/j.scitotenv.2006.10.026. Epub 2006 Nov 29.
Discharge from sewage works has been shown to be an important source of phthalates into the environment which is of major concern because some are toxic, suspected endocrine disruptors and recalcitrant. Laboratory trickle filter microcosms (continuous and re-circulating flow) were constructed and operated to investigate the biodegradation and adsorption of phthalates and also to isolate phthalate degrading microorganisms. It was found that adsorption was critical for the removal of both DEP (77.5%) and DEHP (55.7%) in continuous flow microcosms. The proportion of phthalates removed by biodegradation in the continuous flow microcosms was estimated. Re-circulating flow microcosms improved the removal of DEHP compared to continuous flow microcosms. Microcosm biofilm used for an enrichment culture on phthalate media isolated a varied group of microbes including Gram negative and Gram positive bacteria, yeasts and fungi. Bacteria species with all the necessary enzymes to degrade phthalic acid were isolated.
污水处理厂的排放已被证明是邻苯二甲酸盐进入环境的一个重要来源，这是一个主要关注点，因为一些邻苯二甲酸盐有毒，是疑似内分泌干扰物且具有难降解性。构建并运行了实验室滴滤式微宇宙（连续和循环流动），以研究邻苯二甲酸盐的生物降解和吸附，并分离邻苯二甲酸盐降解微生物。研究发现，在连续流动微宇宙中，吸附对于DEP（77.5%）和DEHP（55.7%）的去除至关重要。估算了连续流动微宇宙中通过生物降解去除的邻苯二甲酸盐比例。与连续流动微宇宙相比，循环流动微宇宙提高了DEHP的去除率。用于在邻苯二甲酸盐培养基上进行富集培养的微宇宙生物膜分离出了不同种类的微生物，包括革兰氏阴性菌、革兰氏阳性菌、酵母和真菌。分离出了具有降解邻苯二甲酸所需全部酶的细菌种类。
