Gauntt C J, Griffith M M, Sauck J R, Upson R H, Carlson E C
J Virol. 1975 Nov;16(5):1265-72. doi: 10.1128/JVI.16.5.1265-1272.1975.
Isopycnic centrifugation of rhinovirus type 14 (RV14), purified from infected HeLa or KB cell cultures, into CsCl gradients resolved two bands of infectious virus particles with buoyant density values of 1.409 +/- 0.007 (H virus) and 1.386 +/- 0.004 (L virus) g/ml. Only H virus was detected by incorporation of radiolabeled uridine into viral RNA, and H virus accounted for the majority of infectivity in gradients. H and L virus could not be differentiated by plaque morphology, extent of neutralization by RV14-specific antiserum, or particle size. Electron microscope studies showed that most L-virus particles were associated with an amorphous material. Treatment of L virus with proteolytic enzymes or rebanding L virus in CsCl gradients resulted in recovery of the majority of infectivity as H virus. Virus purified from cell-free fluids from infected HeLa or KB cell cultures banded only as H virus. HeLa cell cultures challenged with purified H virus and harvested at 3 h postinoculation for virus purification yielded only infectious H virus. Both H and L viruses were detected in cell cultures that had been challenged with purified H virus and harvested at 12 h postinoculation. The data suggest that H virus represents progeny virus, whereas L virus represents sequestered infectious virus particles which become associated with an amorphous material and do not enter into viral replicative processes.
从感染的HeLa或KB细胞培养物中纯化出的14型鼻病毒（RV14），在氯化铯梯度中进行等密度离心，分离出两条具有感染性病毒颗粒的条带，其浮力密度值分别为1.409±0.007（H病毒）和1.386±0.004（L病毒）g/ml。通过将放射性标记的尿苷掺入病毒RNA仅检测到H病毒，并且H病毒在梯度中占感染性的大部分。H病毒和L病毒不能通过噬斑形态、RV14特异性抗血清的中和程度或颗粒大小来区分。电子显微镜研究表明，大多数L病毒颗粒与一种无定形物质相关。用蛋白水解酶处理L病毒或在氯化铯梯度中对L病毒进行再分级，导致大部分感染性恢复为H病毒。从感染的HeLa或KB细胞培养物的无细胞液中纯化的病毒仅以H病毒形式形成条带。用纯化的H病毒攻击HeLa细胞培养物，并在接种后3小时收获用于病毒纯化，仅产生感染性H病毒。在用纯化的H病毒攻击并在接种后12小时收获的细胞培养物中检测到H病毒和L病毒。数据表明，H病毒代表子代病毒，而L病毒代表被隔离的感染性病毒颗粒，这些颗粒与无定形物质相关，不进入病毒复制过程。
