Wallace W E, Keane M J, Hill C A, Xu J, Ong T M
J Toxicol Environ Health. 1987;21(1-2):163-71. doi: 10.1080/15287398709531009.
Diesel exhaust particulate material from exhaust pipe scrapings of two trucks, diluted automobile diesel exhaust particulate material collected on filters, and two oil shale ores were prepared for the Ames mutagenicity assay by dichloromethane (DCM) extraction, by dispersion into 0.85% saline, or by dispersion into dipalmitoyl lecithin (DPL) emulsion in saline. Salmonella typhimurium TA98 was used to detect frameshift mutagens in the samples. Samples of diesel soot gave positive mutagenic responses with both DCM extraction and DPL dispersion, with the DPL dispersion giving higher results in some cases. The results suggest that possible mutagens associated with inhaled particles may be dispersed or solubilized into the phospholipid component of pulmonary surfactant and become active in such a phase.
从两辆卡车的排气管刮屑中获取的柴油机废气颗粒物质、收集在过滤器上的稀释汽车柴油机废气颗粒物质以及两种油页岩矿石，通过二氯甲烷（DCM）萃取、分散到0.85%盐水中或分散到盐水中的二棕榈酰卵磷脂（DPL）乳液中，制备用于Ames致突变性试验的样本。鼠伤寒沙门氏菌TA98用于检测样本中的移码诱变剂。柴油机烟灰样本在DCM萃取和DPL分散处理时均给出了阳性诱变反应，在某些情况下，DPL分散处理给出的结果更高。结果表明，与吸入颗粒相关的潜在诱变剂可能会分散或溶解到肺表面活性剂的磷脂成分中，并在该相中变得活跃。
