沙特超重和肥胖患者中血管紧张素转换酶DD基因型频率增加。

Increased frequency of angiotensin-converting enzyme DD genotype in Saudi overweight and obese patients.

作者信息

El-Hazmi Mohsen Af, Warsy Arjumand S

机构信息

Department of Medical Biochemistry and the WHO Collaborating Centre, College of Medicine, King Khalid University Hospital and Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia,

出版信息

Ann Saudi Med. 2003 Jan-Mar;23(1-2):24-7. doi: 10.5144/0256-4947.2003.24.

DOI:10.5144/0256-4947.2003.24

PMID:17146217

Abstract

BACKGROUND

Several studies have been carried out to investigate the insertion/deletion (I/D) polymorphism of angiotensin-converting enzyme (ACE) in obese and normal weight individuals, often with contradictory results in different populatios. In some studies, the DD genotype occurs at a high frequency in obesity, while in others no association has been demonstrated. Since obesity and overweight are of frequent occurrence in the Saudi population, we initiated this study to investigate the I/D polymorphism of ACE in obese, overweight and normal weight Saudis.

PATIENTS AND METHODS

The study group included 457 Saudi males and females. The height and weight were measured and body mass index (BMI) was calculated. Based on the BMI value, the total study population was classified as normal, overweight and obese. A total of 117 obese (BMI >30 (kg/m(2))), 185 overweight (BMI=25-29.9 (kg/m(2))) and 155 normal weight individuals (BMI< (kg/m (2)) were enrolled. Polymerase chain reaction (PCR) and agarose gel electrophoresis were used to study the ACE polymorphism. For the deletion (D) form, a 190 bp and for the insertion (I) form, 490 bp DNA fragment were obtained on 2% agarose gel electrophoresis.

RESULTS

The frequency of DD genotype was 76.9% in the obese, and 73.5% in the overweight individuals, compared to 19.66% and 24.86% of the ID genotype in the obese and overweight, respectively. Among the normal weight individuals, the frequency was significantly lower for DD and higher for the ID genotype, i.e., 58.7% for DD and 40% for ID. The allele frequencies in the obese were 0.867 and 0.133 for the D and I alleles, respectively. While in the overweight, the frequencies were 0.859 and 0.141 for these alleles, respectively, compared to 0.787 and 0.213 in the normal weight individuals.

CONCLUSIONS

The DD genotype and D allele occur at a high frequency in Saudi overweight and obese individuals, and may have some role in fat accumulation by affecting metabolic pathways of fat, thus leading to the development of overweight and obesity.

摘要

背景

已经开展了多项研究来调查肥胖个体和正常体重个体中血管紧张素转换酶（ACE）的插入/缺失（I/D）多态性，不同人群的研究结果常常相互矛盾。在一些研究中，DD基因型在肥胖人群中出现的频率较高，而在另一些研究中则未发现关联。由于沙特人群中肥胖和超重现象较为常见，我们开展了这项研究，以调查肥胖、超重和正常体重沙特人中ACE的I/D多态性。

患者与方法

研究组包括457名沙特男性和女性。测量身高和体重并计算体重指数（BMI）。根据BMI值，将整个研究人群分为正常、超重和肥胖。共纳入117名肥胖者（BMI>30(kg/m²)）、185名超重者（BMI=25-29.9(kg/m²)）和155名正常体重个体（BMI<(kg/m²)）。采用聚合酶链反应（PCR）和琼脂糖凝胶电泳研究ACE多态性。对于缺失（D）型，在2%琼脂糖凝胶电泳上获得190bp的DNA片段，对于插入（I）型，获得490bp的DNA片段。

结果

肥胖者中DD基因型的频率为76.9%，超重者中为73.5%，而肥胖者和超重者中ID基因型的频率分别为19.66%和24.86%。在正常体重个体中，DD基因型的频率显著较低，ID基因型的频率较高，即DD为58.7%，ID为40%。肥胖者中D和I等位基因的频率分别为0.867和0.133。超重者中这些等位基因的频率分别为0.859和0.141，而正常体重个体中为0.787和0.213。