Alshammary Amal F, Khan Imran Ali
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
J Obes Metab Syndr. 2021 Mar 30;30(1):63-71. doi: 10.7570/jomes20086.
Consanguinity, defined as a blood relation between couples, is associated with genetic diseases in their offspring. In Saudi Arabia, obesity is considered a major health problem associated with increased risks of cardiovascular disease, insulin resistance, and type 2 diabetes mellitus. Angiotensin-converting enzyme () with insertion (I) and deletion (D) polymorphisms of an Alu-287 bp sequence has been implicated in multiple metabolic disorders, including obesity. To date, no studies have been conducted in the Saudi population regarding the gene in consanguineous offspring with obesity. Therefore, the present study aimed to investigate genetic associations in offspring of first cousins, and specifically the relationship between obesity and (Alu-287 bp) was evaluated in the Saudi population.
In total, 91 cases of obesity in the offspring of first-cousin couples and 100 control subjects without obesity but with a family history of consanguinity were included. Using genomic DNA, ID polymorphisms between the cases and controls were evaluated by polymerase chain reaction.
There were strong differences in the height, weight, and body mass index between the cases and controls (<0.001). A genotype analysis confirmed the strong association with allele frequencies (<0.001; odds ratio, 4.902; 95% confidence interval, 2.867-8.379) when compared between the cases and controls. One-way analysis of variance showed a positive correlation with height and logistic regression (covariance) that could not be strongly correlated (>0.05).
In conclusion, the gene polymorphism was found, through allele frequencies, to be associated with obesity in the offspring of consanguineous first cousins in the Saudi population.
近亲结婚定义为夫妻之间的血缘关系，与他们后代的遗传疾病有关。在沙特阿拉伯，肥胖被认为是一个主要的健康问题，与心血管疾病、胰岛素抵抗和2型糖尿病风险增加有关。血管紧张素转换酶（）具有Alu - 287 bp序列的插入（I）和缺失（D）多态性，与包括肥胖在内的多种代谢紊乱有关。迄今为止，尚未在沙特人群中针对肥胖近亲后代中的基因进行研究。因此，本研究旨在调查近亲第一代后代中的基因关联，特别是在沙特人群中评估肥胖与（Alu - 287 bp）之间的关系。
总共纳入了91例近亲夫妻后代中的肥胖病例和100例无肥胖但有近亲家族史的对照受试者。使用基因组DNA，通过聚合酶链反应评估病例组和对照组之间的ID多态性。
病例组和对照组在身高、体重和体重指数方面存在显著差异（<0.001）。基因型分析证实，与对照组相比，病例组在等位基因频率方面存在显著关联（<0.001；优势比，4.902；95%置信区间，2.867 - 8.379）。单因素方差分析显示与身高呈正相关，逻辑回归（协方差）显示两者之间无强相关性（>0.05）。
总之，通过等位基因频率发现，基因多态性与沙特人群中近亲第一代后代的肥胖有关。
