Parr Maria K, Geyer Hans, Hoffmann Bernhard, Köhler Karsten, Mareck Ute, Schänzer Wilhelm
Centre for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Carl-Diem-Weg 6, 50933 Cologne, Germany.
Biomed Chromatogr. 2007 Feb;21(2):164-8. doi: 10.1002/bmc.728.
In numerous studies it has been demonstrated that several nutritional supplements contain prohormones not declared on the label. In the current study two products (effervescent tablets) containing high amounts of the 17-methylated anabolic androgenic steroids metandienone (product 1: 16.8 mg/tablet) and stanozolol (product 2: 14.5 mg/tablet) were identified. Additionally in both products norandrostenedione was detected, in product 2 with minor amounts of several other steroids. The substances identified can cause enormous health risks. In addition, the use of the analyzed tablets can lead to positive doping results for metabolites of the respective steroids in sports. This study again shows the insufficient surveillance of the production and trade of dietary supplements. Consumers should be aware of the enormous health and doping risks connected with the use of such products. For GC-MS identification of the analytes the trimethylsilyl derivatives of the steroids and the mixed N-t-butyldimethylsilyl,O-trimethylsilyl derivatives were used. The quantitation of metandienone, norandrostenedione, and stanozolol was performed using HPLC-DAD.
在众多研究中已证实,几种营养补充剂含有标签上未标明的前体激素。在当前研究中,鉴定出了两种含有大量17 - 甲基化合成代谢雄激素类固醇美雄酮(产品1:16.8毫克/片)和司坦唑醇(产品2:14.5毫克/片)的产品(泡腾片)。此外,在这两种产品中均检测到了去甲雄烯二酮,在产品2中还检测到少量其他几种类固醇。所鉴定出的物质会造成巨大的健康风险。此外,使用所分析的片剂会导致在体育赛事中相应类固醇的代谢产物出现兴奋剂检测阳性结果。这项研究再次表明对膳食补充剂生产和贸易的监管不足。消费者应意识到使用此类产品所带来的巨大健康和兴奋剂风险。为了通过气相色谱 - 质谱联用(GC - MS)鉴定分析物,使用了类固醇的三甲基硅烷基衍生物以及混合的N - 叔丁基二甲基硅烷基、O - 三甲基硅烷基衍生物。美雄酮、去甲雄烯二酮和司坦唑醇的定量分析采用高效液相色谱 - 二极管阵列检测法(HPLC - DAD)。