Petcu Ileana, Savu Diana, Thierens Hubert, Nagels Guy, Vral Anne
Department of Environmental and Life Physics, National Institute for Physics and Nuclear Engineering Horia Hulubei, Bucharest, Romania.
Int J Radiat Biol. 2006 Nov;82(11):793-803. doi: 10.1080/09553000600932976.
To assess the in vitro radiosensitivity of peripheral blood lymphocytes of secondary progressive multiple sclerosis (MS) patients in comparison to healthy individuals.
Radiosensitivity of MS patients lymphocytes to in vitro irradiation of 2 Gy 60Co gamma-rays was studied in whole blood cultures and separated peripheral blood mononuclear cell (PBMC) cultures. Chromosomal radiosensitivity was investigated by means of the G0-micronucleus (MN) assay. The radio-induction of micronuclei was also studied in separated subsets of CD4+ (helper) and CD8+ (suppressor) T cells. Apoptosis was analysed in PBMC cultures using fluorescence microscopy techniques.
The spontaneous MN induction was significantly higher in MS patients compared to healthy controls for whole blood and PBMC cultures. After gamma-irradiation of whole blood cultures no difference in radiosensitivity was observed between patients and controls for MN induction. In irradiated PBMC cultures, the CD4+ lymphocytes of MS patients were less radiosensitive compared to healthy controls and this more resistant behaviour increased with increasing illness duration. Radiation induced apoptosis in G0 lymphocytes of MS patients was lower than in controls.
After gamma irradiation, a radioresistant behaviour in PBMC cultures of MS patients was revealed. This radioresistant behaviour was expressed by lower MN induction in CD4+ lymphocytes and by lower apoptosis induction in G0 PBMC cultures and may point to an adaptive response. The higher radiation response in whole blood cultures compared to PBMC cultures was significantly more pronounced in MS patients, suggesting special characteristics of the whole blood environment associated with this pathology.
评估继发进展型多发性硬化症（MS）患者外周血淋巴细胞与健康个体相比的体外放射敏感性。
在全血培养物和分离的外周血单核细胞（PBMC）培养物中研究MS患者淋巴细胞对2 Gy 60Coγ射线体外照射的放射敏感性。通过G0微核（MN）试验研究染色体放射敏感性。还在CD4 +（辅助）和CD8 +（抑制）T细胞的分离亚群中研究了微核的放射诱导。使用荧光显微镜技术在PBMC培养物中分析细胞凋亡。
对于全血和PBMC培养物，MS患者的自发MN诱导明显高于健康对照。全血培养物经γ射线照射后，患者和对照在MN诱导方面未观察到放射敏感性差异。在照射的PBMC培养物中，MS患者的CD4 +淋巴细胞与健康对照相比放射敏感性较低，并且这种更具抗性的行为随着病程的增加而增加。MS患者G0淋巴细胞中辐射诱导的细胞凋亡低于对照。
γ射线照射后，MS患者的PBMC培养物中显示出放射抗性行为。这种放射抗性行为表现为CD4 +淋巴细胞中MN诱导较低以及G0 PBMC培养物中细胞凋亡诱导较低，可能表明存在适应性反应。与PBMC培养物相比，全血培养物中更高的辐射反应在MS患者中更为明显，表明与这种病理相关的全血环境具有特殊特征。
