Valdiosera Cristina, García Nuria, Dalén Love, Smith Colin, Kahlke Ralf-Dietrich, Lidén Kerstin, Angerbjörn Anders, Arsuaga Juan Luis, Götherström Anders
Centro UCM-ISCIII de Evolución y Comportamiento Humanos, C/Sinesio Delgado 4, 28029 Madrid, Spain.
Biol Lett. 2006 Dec 22;2(4):601-3. doi: 10.1098/rsbl.2006.0515.
In this study, we have used a technique designed to target short fragments containing informative mitochondrial substitutions to extend the temporal limits of DNA recovery and study the molecular phylogeny of Ursus deningeri. We present a cladistic analysis using DNA recovered from 400 kyr old U. deningeri remains, which demonstrates U. deningeri's relation to Ursus spelaeus. This study extends the limits of recovery from skeletal remains by almost 300 kyr. Plant material from permafrost environments has yielded DNA of this age in earlier studies, and our data suggest that DNA in teeth from cave environments may be equally well preserved.
在本研究中，我们使用了一种技术，该技术旨在靶向包含信息丰富的线粒体替代的短片段，以扩展DNA恢复的时间范围，并研究德宁格熊（Ursus deningeri）的分子系统发育。我们使用从40万年历史的德宁格熊遗骸中提取的DNA进行了分支分析，这表明了德宁格熊与洞穴熊（Ursus spelaeus）的关系。这项研究将从骨骼遗骸中恢复DNA的时间范围延长了近30万年。在早期研究中，来自永久冻土环境的植物材料已产生了这个年代的DNA，而我们的数据表明，洞穴环境中牙齿里的DNA可能保存得同样完好。
