Wu Wei, Liang Zhitao, Zhao Zhongzhen, Cai Zongwei
Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong SAR, China.
J Mass Spectrom. 2007 Jan;42(1):58-69. doi: 10.1002/jms.1138.
A method for the direct determination of alkaloid profiling in plant tissues by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was developed. The alkaloid profiles of the herbs were obtained without the need for complicated sample preparation. Experimental results demonstrated that the direct MALDI-TOFMS analysis allowed rapid and reliable characterization of the components in plant tissues. Four commonly used Chinese medicinal herbs were studied, including Aconitum Carmichaeli Debx. (Fuzi in Chinese) and Processed Fuzi, for herb differentiation and explanation of the significant difference in their toxicities. The direct analysis method proved valuable for the preliminary study of plant component profiles. The rapid collection of information from the direct analysis on plant tissues could be valuable for supporting the discovery of new compounds and for the quality control of medicinal herbs.
开发了一种利用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）直接测定植物组织中生物碱谱的方法。无需复杂的样品制备即可获得草药的生物碱谱。实验结果表明，直接MALDI-TOFMS分析能够快速、可靠地表征植物组织中的成分。研究了四种常用中药材，包括附子（中药名）和炮附子，用于草药鉴别以及解释它们毒性的显著差异。该直接分析方法被证明对植物成分谱的初步研究具有重要价值。从对植物组织的直接分析中快速收集信息，对于支持新化合物的发现和草药质量控制可能具有重要价值。
