Yue Hao, Pi Zifeng, Song Fengrui, Liu Zhiqiang, Cai Zongwei, Liu Shuying
Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, China.
Talanta. 2009 Mar 15;77(5):1800-7. doi: 10.1016/j.talanta.2008.10.022. Epub 2008 Oct 22.
Studies of aconitine-type alkaloids in the Chinese herb Aconitum Carmichaeli were performed by HPLC/ESIMS/MS(n) and FTICR/ESIMS in positive ion mode. The characteristic fragmentation pathways in the MS(n) spectra were summarized based on previously published research literature and further study. According to the fragmentation pathways of mass spectrometry, results from the analysis of standard compounds and reports from literature, 111 compounds were identified or deduced in a total of 117 found compounds in A. Carmichaeli. In the 11 monoester-diterpenoid alkaloids (MDA), 10 diesterditerpenoid alkaloids (DDA) and 81 lipo-alkaloids, the novel alkaloids including 1 MDA, 2 DDA and 48 lipo-alkaloids were detected. In addition, 1 DDA, 7 lipo-alkaloids and 2 alkaloids with small molecular weights that possess C19-norditerpenoid skeleton were reported in A. Carmichaeli for the first time.
采用高效液相色谱/电喷雾离子化串联质谱（HPLC/ESIMS/MS(n)）和傅里叶变换离子回旋共振质谱/电喷雾离子化（FTICR/ESIMS）在正离子模式下对中药乌头中的乌头碱型生物碱进行了研究。基于先前发表的研究文献和进一步研究，总结了MS(n)谱中的特征性裂解途径。根据质谱裂解途径、标准化合物分析结果及文献报道，在乌头中总共发现的117种化合物中，鉴定或推断出了111种化合物。在11种单酯二萜生物碱（MDA）、10种双酯二萜生物碱（DDA）和81种脂生物碱中，检测到了包括1种MDA、2种DDA和48种脂生物碱在内的新生物碱。此外，首次在乌头中报道了1种DDA、7种脂生物碱和2种具有C19-去甲二萜骨架的小分子生物碱。
