含4'-O-硝基苄氧基胸腺嘧啶的寡核苷酸的合成及其反应活性。

Synthesis of oligonucleotide containing 4'-o-nitrobenzyloxythymidine and its reactivity.

作者信息

Aso Mariko, Fukuda Mitsuhiro, Usui Kazuteru, Suemune Hiroshi

机构信息

Graduate School of Pharmaceutical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2004(48):59-60. doi: 10.1093/nass/48.1.59.

DOI:10.1093/nass/48.1.59

PMID:17150477

Abstract

We synthesized oligonucleotide (1) containing 4'-o-nitrobenzyloxythymidine (2), which was prepared by reaction of 4',5'-unsaturated thymidine with m-CPBA followed by Lewis-acid catalyzed addition of o-nitrobenzylalcohol. The duplex stability of 1 with the complementary oligonucleotide was studied by Tm measurement. Photochemical reaction of 1 to generate C4'-oxidized abasic site containing oligonucleotide (3) was studied.

摘要

我们合成了含有4'-邻硝基苄氧基胸苷（2）的寡核苷酸（1），4'-邻硝基苄氧基胸苷（2）是通过4',5'-不饱和胸苷与间氯过氧苯甲酸反应，然后在路易斯酸催化下加入邻硝基苄醇制备的。通过测量熔解温度（Tm）研究了1与互补寡核苷酸的双链稳定性。研究了1的光化学反应以生成含C4'-氧化无碱基位点的寡核苷酸（3）。